Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat
Regulation of scavenger receptor, class B, type I, a high density lipoprotein receptor, in liver and steroidogenic tissues of the rat
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DOI：
10.1172/jci118883
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发表时间：
1996-08-15
影响因子：
15.9
通讯作者：
Hobbs, HH
中科院分区：
文献类型：
--
作者：
Landschulz, KT;Pathak, RK;Hobbs, HH
清道夫受体，B类，I型（SR-BI）结合HDL，并介导胆固醇酯从HDL到培养细胞的选择性转移。SR-BI在小鼠中的组织分布表明，该受体可将HDL-胆固醇递送到肝脏和非胎盘类固醇生成组织。为了研究SR-BI在体内的作用，我们确定了其在大鼠中的组织和细胞类型特异性表达模式和调节。高水平的免疫可检测的SR-BI存在于肾上腺、卵巢和肝脏中。SR-BI通过免疫荧光定位于肾上腺的束状核和网状核中的类固醇生成细胞的表面和卵巢的黄体细胞。高剂量雌激素处理显著降低了肝脏中的SR-BI，增加了肾上腺和卵巢黄体细胞中的SR-BI。这些雌激素诱导的肾上腺和卵巢中SR-BI的增加伴随着体内HDL对荧光脂质的摄取增强。给药人绒毛膜促性腺激素诱导睾丸类固醇生成间质细胞中SR-BI显著增加。这些结果表明，SR-BI介导体内从HDL到非胎盘类固醇生成组织的胆固醇生理相关摄取。
The scavenger receptor, class B, type I(SR-BI) binds HDL and mediates the selective transfer of cholesteryl esters from HDL to cultured cells, The tissue distribution of SR-BI in mice suggests that this receptor may deliver HDL-cholesterol to the liver and to nonplacental steroidogenic tissues. To examine the role of SR-BI in vivo, we determined its tissue and cell type-specific expression pattern and regulation in rats, High levels of immunodetectable SR-BI were present in the adrenal gland, ovary, and liver, In pregnant animals, the mammary gland also expressed high levels of the protein. SR-BI was localized by immunofluorescence to the surfaces of steroidogenic cells in the zona fasciculata and zona reticularis of the adrenal gland and to the corpus luteal cells of the ovary. High-dose estrogen treatment dramatically reduced SR-BI in the liver and increased SR-BI in the adrenal gland and corpus luteal cells of the ovary, These estrogen-induced increases in SR-BI in the adrenal gland and ovary were accompanied by enhanced in vivo uptake of fluorescent lipid from HDL. The administration of human chorionic gonadotropin induced a dramatic increase in SR-BI in the steroidogenic Leydig cells of the testes, These findings suggest that SR-BI mediates physiologically relevant uptake of cholesterol from HDL to nonplacental steroidogenic tissues in vivo.