Selective bactericidal activity of nanopatterned superhydrophobic cicada Psaltoda claripennis wing surfaces
Selective bactericidal activity of nanopatterned superhydrophobic cicada Psaltoda claripennis wing surfaces
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DOI：
10.1007/s00253-012-4628-5
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发表时间：
2013-10-01
影响因子：
5
通讯作者：
Ivanova, Elena P.
中科院分区：
文献类型：
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作者：
Hasan, Jafar;Webb, Hayden K.;Ivanova, Elena P.
Clanger Clanger（Psaltoda claripennis）翅膀表面的纳米图案代表了一类新的生物材料的第一个例子，这种生物材料可以仅仅基于其物理表面结构在接触时杀死细菌。因此，它们提供了一种用于开发新型功能表面的模型，该功能表面具有对细菌污染和感染的增加的抗性。然而，它们对广谱细菌的有效性尚未确定。在这里，针对几种细菌物种测试了翅膀的杀菌特性，这些细菌物种具有一系列形态和细胞壁类型的组合。供试菌种主要为病原体，包括枯草芽孢杆菌、卡他布兰汉菌、大肠埃希菌、海洋动球菌、铜绿假单胞菌、荧光假单胞菌和金黄色葡萄球菌。发现翅膀一致地杀死革兰氏阴性细胞（即，B。卡他炎、E.大肠杆菌、铜绿假单胞菌和荧光假单胞菌），而革兰氏阳性细胞（B. subtilis、P. maritimus和S.金黄色葡萄球菌）保持抗性。细胞的形态似乎在确定细胞易感性方面不起任何作用。翅膀的杀菌活性也被发现是相当有效的;在孵育30分钟后，每平方厘米翅膀表面的悬浮液中有6.1 +/- 1.5 × 10（6）个铜绿假单胞菌细胞被灭活。这些发现证明了将双翅纳米粒子结合到设计中的选择性杀菌表面的发展潜力。
The nanopattern on the surface of Clanger cicada (Psaltoda claripennis) wings represents the first example of a new class of biomaterials that can kill bacteria on contact based solely on its physical surface structure. As such, they provide a model for the development of novel functional surfaces that possess an increased resistance to bacterial contamination and infection. Their effectiveness against a wide spectrum of bacteria, however, is yet to be established. Here, the bactericidal properties of the wings were tested against several bacterial species, possessing a range of combinations of morphology and cell wall type. The tested species were primarily pathogens, and included Bacillus subtilis, Branhamella catarrhalis, Escherichia coli, Planococcus maritimus, Pseudomonas aeruginosa, Pseudomonas fluorescens, and Staphylococcus aureus. The wings were found to consistently kill Gram-negative cells (i.e., B. catarrhalis, E. coli, P. aeruginosa, and P. fluorescens), while Gram-positive cells (B. subtilis, P. maritimus, and S. aureus) remained resistant. The morphology of the cells did not appear to play any role in determining cell susceptibility. The bactericidal activity of the wing was also found to be quite efficient; 6.1 +/- 1.5 x 10(6) P. aeruginosa cells in suspension were inactivated per square centimeter of wing surface after 30-min incubation. These findings demonstrate the potential for the development of selective bactericidal surfaces incorporating cicada wing nanopatterns into the design.