Prolonged Thrombocytopenia Following Allogeneic Hematopoietic Stem Cell Transplantation and Its Association with a Reduction in Ploidy and an Immaturation of Megakaryocytes
Prolonged Thrombocytopenia Following Allogeneic Hematopoietic Stem Cell Transplantation and Its Association with a Reduction in Ploidy and an Immaturation of Megakaryocytes
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同种异体造血干细胞移植后长期血小板减少及其与倍性减少和巨核细胞不成熟的关系
DOI：
10.1016/j.bbmt.2010.09.007
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发表时间：
2011-02-01
影响因子：
4.3
通讯作者：
Huang, Xiaojun
中科院分区：
文献类型：
--
作者：
Zhang, Xiaohui;Fu, Haixia;Huang, Xiaojun
持续性血小板减少症是异基因造血干细胞移植（allo-HSCT）后常见的并发症，但其发病机制尚不清楚。在本研究中，我们使用流式细胞术来确定骨髓巨核细胞（MK）的频率和MK倍体分布在异基因造血干细胞移植受者或没有长期血小板减少症（n = 32和27，分别）和健康志愿者（n = 13）。此外，还测定了MK中c-Mpl的表达。结果表明：3组间骨髓单个核细胞中MKs的比例及CD 110（+）MKs占总MKs的百分比无显著性差异;然而，在非血小板减少的allo-HSCT受者与健康志愿者的比较中，长期血小板减少的allo-HSCT患者表现出明显的向低倍体细胞的转变（左移），同时伴随着
Prolonged thrombocytopenia is a frequent complication after allogeneic hematopoietic stem cell transplantation (allo-HSCT); however, its pathogenesis has remained obscure. In the present study, we used flow cytometry to determine the frequency of bone marrow megakaryocytes (MKs) and MK ploidy distributions in allo-HSCT recipients with or without prolonged thrombocytopenia (n = 32 and 27, respectively) and healthy volunteers (n = 13). In addition, the expression of c-Mpl in MKs was measured. The results indicate that the proportions of MKs in marrow mononuclear cells or the percentages of CD 110(+) MKs in total MKs did not significantly differ between the 3 groups; however, in a comparison of nonthrombocytopenic allo-HSCT recipients to healthy volunteers, the allo-HSCT patients who had prolonged thrombocytopenia exhibited significant shifts toward low ploidy cells (left shift), which were accompanied by a marked increase in