Amplification and overexpression of E2F3 in human bladder cancer
Amplification and overexpression of E2F3 in human bladder cancer
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DOI：
10.1038/sj.onc.1207274
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发表时间：
2004-02-26
期刊：
影响因子：
8
通讯作者：
Cooper, CS
中科院分区：
文献类型：
--
作者：
Feber, A;Clark, J;Cooper, CS
我们证明，在人膀胱癌中，位于6p22的E2F3基因的扩增与其编码的mRNA转录产物的过度表达和E2F3蛋白的高水平表达有关。对E2F3蛋白水平的免疫组织化学分析表明，约三分之一(33/101)的原发性膀胱移行细胞癌过表达核E2F3蛋白，且核E2F3过表达的肿瘤所占比例随肿瘤分期和分级的增加而增加。考虑到E2F3在细胞周期进程中的作用，这些结果表明E2F3基因代表了一个候选的膀胱癌基因，该基因由DNA扩增和过度表达激活。
We demonstrate that, in human bladder cancer, amplification of the E2F3 gene, located at 6p22, is associated with overexpression of its encoded mRNA transcripts and high levels of expression of E2F3 protein. Immunohistochemical analyses of E2F3 protein levels have established that around one-third (33/101) of primary transitional cell carcinomas of the bladder overexpress nuclear E2F3 protein, with the proportion of tumours containing over-expressed nuclear E2F3 increasing with tumour stage and grade. When considered together with the established role of E2F3 in cell cycle progression, these results suggest that the E2F3 gene represents a candidate bladder cancer oncogene that is activated by DNA amplification and overexpression.