Wound healing effect of electrospun silk fibroin nanomatrix in burn-model
Wound healing effect of electrospun silk fibroin nanomatrix in burn-model
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DOI：
10.1016/j.ijbiomac.2015.12.055
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发表时间：
2016-04-01
影响因子：
8.2
通讯作者：
Park, Chan Hum
中科院分区：
文献类型：
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作者：
Ju, Hyung Woo;Lee, Ok Joo;Park, Chan Hum
丝素蛋白是近年来在组织工程中应用的重要生物材料。本研究采用静电纺丝法制备丝素纳米基质，并对其作为烧伤大鼠模型创面敷料进行评价。观察创面缩小、组织学检查、转化生长因子tgf - β 1和白细胞介素IL-1 α、6、10的定量测定，评价创面愈合效果。丝素纳米基质处理在减少创面大小和上皮化方面表现出有效的效果。组织学结果还显示，丝素纳米基质处理组真皮中胶原蛋白的沉积是通过覆盖创面来组织的。在烧伤后7天，丝素纳米基质处理后的损伤皮肤中促炎细胞因子（IL-1 α）的表达水平明显低于医用纱布（对照组）。丝素纳米基质创面tgf - β 1表达在创面处理后21天达到峰值，而纱布组创面处理后第7天tgf - β 1表达最高。综上所述，丝素蛋白纳米基质具有促进烧伤创面愈合的作用，是一种很好的烧伤创面治疗材料。(C) 2015 Elsevier B.V.版权所有
Silk fibroin has recently become an important biomaterial for tissue engineering application. In this study, silk fibroin nanomatrix was fabricated by electrospinning and evaluated as wound dressing material in a burn rat model. The wound size reduction, histological examination, and the quantification of transforming growth factor TGF-beta 1 and interleukin IL-1 alpha, 6, and 10 were measured to evaluate the healing effects. The silk fibroin nanomatrix treatment exhibited effective performance in decreasing the wound size and epithelialization. Histological finding also revealed that the deposition of collagen in the dermis was organized by covering the wound area in the silk fibroin nanomatrix treated group. The expression level of pro-inflammatory cytokine (IL-1 alpha) was significantly reduced in the injured skin following the silk fibroin nanomatrix treatment compared to the medical gauze (control) at 7 days after burn. Also, the expression level of TGF-beta 1 in the wound treated with silk fibroin nanomatrix peaked 21-days post-treatment whereas expression level of TGF-beta 1 was highest at day 7 in the gauze treated group. In conclusion, this data demonstrates that silk fibroin nanomatrix enhances the burn wound healing, suggesting it is a good candidate for burn wound treatment. (C) 2015 Elsevier B.V. All rights reserved.