Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour
Hydrogen sulfide-producing bacteria in tongue biofilm and their relationship with oral malodour
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DOI：
10.1099/jmm.0.46118-0
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发表时间：
2005-09-01
影响因子：
3
通讯作者：
Takahashi, N
中科院分区：
文献类型：
--
作者：
Washio, J;Sato, T;Takahashi, N
本研究的目的是鉴定舌生物膜微植物群中产生硫化氢（H2S）的细菌，并探讨细菌植物群与口腔空气中H2S水平的关系。10名受试者（年龄21-56岁）的口腔异味水平进行了评估，气相色谱法，Breathtron和感官评分。根据这些评估，受试者被分为两组：气味组和无/低气味组。对舌苔进行取样，并涂布在含有0（.）05%半胱氨酸，0（.）12%谷胱甘肽和0（.）02%乙酸铅，然后在37 ℃厌氧培养2周。选择形成黑色或灰色菌落的细菌作为产生H2S的表型。有气味组的细菌总数（P < 0.005）和H2S产生菌数（P < 0.05）均显著高于无气味组和低气味组。对形成黑色或灰色菌落的细菌（1126株来自气味组; 242株来自无/低气味组）进行传代培养，确认为产生H2S，并根据16 S rRNA基因测序进行鉴定。在有气味和无/低气味组中，Veillonella（38.1%;无/低气味组中46.3%）、放线菌（25.4%; 17.7%）和Prevotella（10.3%; 7.8%）是主要的H2S产生菌。这些结果表明，舌头生物膜中产生H2S的细菌数量的增加是口腔异味的原因，尽管两组之间舌头生物膜的细菌组成相似。
The aims of this study were to identify hydrogen sulfide (H2S)-producing bacteria among tongue biofilm microflora and to investigate the relationship between bacterial flora and H2S levels in mouth air. Oral malodour levels in 10 subjects (age 21-56 years) were assessed by gas chromatography, and Breathtron and organoleptic scores. Based on these assessments, subjects were divided into two groups: an odour group and a no/low odour group. Tongue coatings were sampled and spread onto Fastidious Anaerobe Agar plates containing 0(.)05 % cysteine, 0(.)12 % glutathione and 0(.)02 % lead acetate, and were then incubated anaerobically at 37 degrees C for 2 weeks. Bacteria forming black or grey colonies were selected as H2S-producing phenotypes. The numbers of total bacteria (P < 0.005) and H2S-producing bacteria (P < 0.05) in the odour group were significantly larger than those in the no/low odour group. Bacteria forming black or grey colonies (1126 isolates from the odour group; 242 isolates from the no/low odour group) were subcultured, confirmed as producing H2S and identified according to 16S rRNA gene sequencing. Species of Veillonella (38.1 % in odour group; 46.3 % in no/low odour group), Actinomyces (25.4 %; 17.7 %) and Prevotella (10.3 %; 7.8 %) were the predominant H2S-producing bacteria in both the odour and no/low odour groups. These results suggest that an increase in the number of H2S-producing bacteria in the tongue biofilm is responsible for oral malodour, although the bacterial composition of tongue biofilm was similar between the two groups.