Enteral supplement enriched with glutamine, fiber, and oligosaccharide attenuates experimental colitis in mice
Enteral supplement enriched with glutamine, fiber, and oligosaccharide attenuates experimental colitis in mice
复制标题
DOI：
10.1016/j.nut.2012.09.007
复制
发表时间：
2013-03-01
期刊：
影响因子：
4.4
通讯作者：
Inagaki, Nobuya
中科院分区：
文献类型：
--
作者：
Joo, Erina;Yamane, Shunsuke;Inagaki, Nobuya
目的：溃疡性结肠炎（Ulcerative colitis，UC）是一种以结肠粘膜急性炎症为特征的慢性复发性疾病.在日本，富含谷氨酰胺、膳食纤维和低聚糖（GFO）的膳食补充剂产品被广泛应用于肠内营养支持。这三种成分被认为可以改善肠道健康。在这项研究中，我们调查是否GFO有抑制作用的粘膜损伤溃疡性结肠炎在实验mouse model.Methods：C57 BL/6小鼠给予2.5%葡聚糖硫酸钠在饮用水5天，诱导结肠炎。然后分别给予GFO或20%葡萄糖溶液0.25mL，每日2次，共10 d。结果：GFO治疗组小鼠的体重减轻和疾病活动指数明显低于葡萄糖治疗组（P < 0.05）。与葡萄糖组相比，GFO治疗组小鼠结肠长度的减少明显减轻（P < 0.01）。此外，组织学结果显示，GFO治疗的小鼠肠道炎症显著减弱。此外，GFO治疗显著抑制葡聚糖硫酸钠诱导的IL-1 β mRNA表达的增加。结论：这些结果表明GFO作为溃疡性结肠炎的辅助治疗具有潜在的治疗价值。(C)2013 Elsevier Inc. All rights reserved.
Objective: Ulcerative colitis is a chronic recurrent disease characterized by acute inflammation of the colonic mucosa. In Japan, a dietary supplementation product enriched with glutamine, dietary fiber, and oligosaccharide (GFO) is widely applied for enteral nutrition support. These three components have been suggested to improve intestinal health. In this study, we investigated whether GFO has suppressive effects on mucosal damage in ulcerative colitis in an experimental mouse model.Methods: C57BL/6 mice received 2.5% dextran sulfate sodium in drinking water for 5 d to induce colitis. Then, they were given 0.25 mL of GFO or a 20% glucose solution twice daily for 10 d. Another set of mice receiving unaltered drinking water was used as the normal control group.Results: The body weight loss and disease activity index were significantly lower in the GFO-treated mice compared with the glucose-treated mice (P < 0.05). The decrease in colon length induced by dextran sulfate sodium was significantly alleviated in GFO-treated mice compared with glucose-treated mice (P < 0.01). In addition, the histologic findings showed that intestinal inflammation was significantly attenuated in mice treated with GFO. Furthermore, treatment with GFO significantly inhibited the dextran sulfate sodium-induced increase in the mRNA expression of interleukin-1 beta.Conclusion: These results suggest that GFO has potential therapeutic value as an adjunct therapy for ulcerative colitis. (C) 2013 Elsevier Inc. All rights reserved.