Development of 13 polymorphic chloroplast DNA markers in Quercus gilva, a regionally endemic species in Japan

Development of 13 polymorphic chloroplast DNA markers in Quercus gilva, a regionally endemic species in Japan
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DOI:
10.1007/s12686-014-0256-y
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发表时间:
2014-12-01
影响因子:
1.1
通讯作者:
Ide, Yuji
Ide, Yuji
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Sugiura, Nami;Kurokochi, Hiroyuki;Ide, Yuji

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黄栎(Quercus gilva)是一种万年青栎属,原产于日本、中国大陆和台湾。然而,由于历史上的伐木行为和栖息地的破坏,在古代,Q。在日本的几个地区,gilva面临灭绝的威胁。本研究利用13个多态性标记对Q. gilva是用下一代测序技术开发的。这些标记可以区分两个彼此接近的个体以及来自地理上不同地区的个体。这表明该标记可用于Q的遗传结构和地理分布的研究。gilva的大型和精细规模,可能为我们提供有用的信息,为保护这个物种。
Quercus gilva is an evergreen oak species which is native to Japan, continental China and the islands of Taiwan. However, because of historical logging practices and habitat destruction in ancient times, Q. gilva is threatened with extinction in several regions of Japan. In this study, 13 polymorphic markers spanning the whole chloroplast DNA of Q. gilva were developed, using next-generation sequencing technology. These markers could distinguish between two individuals established in close proximity to one another as well as those from geographically-distinct regions. This indicates that the markers will be suitable for detecting the genetic structure and phylogeography of Q. gilva on both a large and a fine scale, potentially providing us with useful information for the conservation of this species.