Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp
Multipotent mesenchymal stem cells with immunosuppressive activity can be easily isolated from dental pulp
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DOI：
10.1097/01.tp.0000173794.72151.88
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发表时间：
2005-09-27
期刊：
影响因子：
6.2
通讯作者：
Bagnara, GP
中科院分区：
文献类型：
--
作者：
Pierdomenico, L;Bonsi, L;Bagnara, GP
背景骨髓间充质干细胞（MSC）目前正在研究在临床前和临床环境，因为他们的多能分化能力，或者，他们的免疫抑制功能。本研究的目的是评估牙髓（DP）作为替代骨髓（BM）的MSC的潜在来源。流式细胞仪检测结果显示，骨髓间充质干细胞和骨髓间充质干细胞均呈SH 2、SH 3、SH 4、CD 29和CD 166阳性。采用~ 3 H-胸腺嘧啶核苷掺入法测定骨髓间充质干细胞的体外增殖动力学。将PHA刺激的同种异体T细胞与MSCs共培养3 d，检测MSCs的免疫抑制功能。骨髓间充质干细胞在体外可分化为成骨细胞、成软骨细胞和成脂细胞。骨髓间充质干细胞具有成骨和成脂分化能力，但不能分化为软骨细胞。虽然DP-MSCs在体外培养的第3天和第8天之间快速生长，然后在第15天降低其增殖，但BM-MSCs在同一时间段内具有稳定和持续的增殖。通过3 H-胸苷测定法评估，DP-MSCs或BM-MSCs的加入分别导致91 +/- 4%和75 +/- 3%的T细胞应答抑制。牙髓是一种容易获得和有效的MSC来源，与从骨髓中分离的MSC具有不同的动力学和分化潜力。DP-MSCs的快速增殖能力和免疫调节特性可能会促进未来的研究，旨在使用这些细胞治疗或预防造血或实体器官同种异体移植中的T细胞同种异体反应性。
Background. Bone marrow mesenchymal stem cells (MSCs) are currently being investigated in preclinical and clinical settings because of their multipotent differentiative capacity or, alternatively, their immunosuppressive function. The aim of this study was to evaluate dental pulp (DP) as a potential source of MSCs instead of bone marrow (BM).Methods. Flow cytometric analysis showed that DP-MSCs and BM-MSCs were equally SH2, SH3, SH4, CD29 and CD 166 positive. The in vitro proliferative kinetics of MSCs were measured by 3H-thymidine incorporation uptake. The immunosuppressive function of MSCs was then tested by coculturing PHA-stimulated allogeneic T cells with or without MSCs for 3 days.Results. BM-MSCs could be differentiated in vitro into osteogenic, chondrogenic and adipogenic lineages. DP-MSCs showed osteogenic and adipocytic differentiation, but did not differentiate into chondrocytes. Although DP-MSCs grow rapidly in vitro between day 3 and day 8 of culture and then decrease their proliferation by day 15, BM-MSCs have a stable and continuous proliferation over the same period of time. The addition of DP-MSCs or BM-MSCs resulted in 91 +/- 4% and 75 +/- 3% inhibition of T cell response, respectively, assessed by a 3H-thymidine assay.Conclusions. Dental pulp is an easily accessible and efficient source of MSCs, with different kinetics and differentiation potentialities from MSCs as isolated from the bone marrow. The rapid proliferative capacity together with the immunoregulatory characteristics of DP-MSCs may prompt future studies aimed at using these cells in the treatment or prevention of T-cell alloreactivity in hematopoietic or solid organ allogeneic transplantation.