Spectroscopic characterization of nanoErythrosomes in the absence and presence of conjugated polyethyleneglycols:: an FTIR and 31P-NMR study
Spectroscopic characterization of nanoErythrosomes in the absence and presence of conjugated polyethyleneglycols:: an FTIR and 31P-NMR study
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DOI：
10.1016/s0005-2736(02)00465-0
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发表时间：
2002-08-31
影响因子：
3.4
通讯作者：
Auger, M
中科院分区：
文献类型：
--
作者：
Pouliot, R;Saint-Laurent, A;Auger, M
我们最近从红细胞中开发出一种新的输送系统，叫做纳米红体。这些纳米囊泡为生物或非生物化合物的包封以及靶向剂的结合提供了高度的多功能性。特别是，聚乙二醇可以通过共价连接到构成不同蛋白质的基本氨基酸残基上。聚乙烯乙二醇与纳米红血球膜的结合对于这种递送系统的治疗用途可能是有趣的，因为它可以克服异种免疫原性并减少循环中的快速清除。在本研究中，我们研究了温度对纳米红小体在共轭聚乙二醇存在和不存在的情况下行为的影响。更具体地说，傅里叶变换红外（FTIR）光谱已经被用来评估纳米红体在分子量为2000和5000 g mol（-1）的聚乙二醇共价结合后的脂质顺序和动力学、水合作用和蛋白质聚集程度。结果表明，在50℃的温度下，纳米红体的脂链顺序不受显著影响。他们还表明，纳米红小体蛋白在37度以上的温度下不可逆地聚集，这种效应在聚乙二醇的存在下被消除。聚乙二醇的存在降低了水对脂质头基团的可及性。另一方面，p -31核磁共振（NMR）和电子显微镜结果显示，聚乙二醇的存在阻止了纳米红体结构的聚集。(C) 2002 Elsevier Science b.v.版权所有
We have recently developed from red blood cells a new delivery system called nanoErythrosomes. These nanovesicles offer a high degree of versatility for the encapsulation of biological or nonbiological compounds and for the binding of targeting agents. In particular, polyethyleneglycols can be conjugated by a covalent link to the basic amino acid residues constitutive of the different proteins. The binding of polyethyleneglycols to the nanoErythrosorne membrane could be interesting for the therapeutic use of this delivery system since it could overcome heterologous immunogenicity and reduce rapid clearance from circulation. In the present study, we have investigated the effect of temperature on the nanoErythrosome behavior in the absence and presence of conjugated polyethyleneglycols. More specifically, Fourier transform infrared (FTIR) spectroscopy has been used to evaluate the lipid order and dynamics, the hydration and the degree of protein aggregation of the nanoErythrosomes after covalent binding of polyethyleneglycols having molecular weights of 2000 and 5000 g mol(-1). The results indicate that the nanoErythrosome lipid chain order is not significantly affected by heating the nanoErythrosomes at temperatures up to 50 degreesC. They also indicate that the nanoErythrosome proteins aggregate irreversibly at temperatures above 37 degreesC, this effect being abolished in the presence of polyethyleneglycols. The presence of polyethyleneglycols decreases the accessibility of water to the lipid head groups. On the other hand, P-31-nuclear magnetic resonance (NMR) and electron microscopy results reveal that the presence of polyethyleneglycols prevents the aggregation of the nanoErythrosome structures. (C) 2002 Elsevier Science B.V All rights reserved.