ACTIVITY OF L-CARNITINE AND L-ACETYLCARNITINE ON CHOLINOCEPTIVE NEOCORTICAL NEURONS OF THE RAT INVIVO
ACTIVITY OF L-CARNITINE AND L-ACETYLCARNITINE ON CHOLINOCEPTIVE NEOCORTICAL NEURONS OF THE RAT INVIVO
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DOI：
10.1007/bf01250574
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发表时间：
1991-01-01
期刊：
影响因子：
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通讯作者：
TEMPESTA, E
中科院分区：
文献类型：
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作者：
JANIRI, L;FALCONE, M;TEMPESTA, E
肉毒碱和乙酰肉毒碱已被证明存在于中枢神经系统中，并参与胆碱能机制，即使它们在神经传递中的确切作用仍不清楚。本实验在大鼠体感觉皮层单个胆碱感受神经元上进行了微离子电泳研究，目的是分析L-、d -肉碱和L-乙酰肉碱对神经元自发放电和对一些可能的递质的反应的影响。左旋肉碱和l -乙酰左旋肉碱提高了自发放电率，而d -左旋肉碱则无效。l -乙酰肉碱明显增强胆碱能兴奋反应。相反，左旋肉碱在很大程度上降低了单位的胆碱能反应，并抑制了l -乙酰左旋肉碱引起的兴奋反应。阿托品阻断了左旋肉碱和l -乙酰左旋肉碱引起的放电速率的增加，表明两者都具有毒蕈碱活性。肉毒碱与GABA和谷氨酸之间没有相互作用。这些结果表明，肉碱和乙酰肉碱是具有拟胆碱活性的立体特异性神经活性化合物。它们可能在胆碱感觉皮层神经元的调节系统中起作用。
Carnitine and acetylcarnitine have been demonstrated to be present in the CNS and to be involved in cholinergic mechanisms, even if their exact role in neurotransmission is still unknown.This microiontophoretic study was carried out on single cholinoceptive neurons of the somatosensory cortex in the rat in order to analyze the effects of L- and D-carnitine and L-acetylcarnitine on the spontaneous firing and the neuronal responses to some putative transmitters.L-carnitine and L-acetylcarnitine increased the spontaneous discharge rate, while D-carnitine was found to be ineffective. L-acetylcarnitine clearly potentiated the cholinergic excitatory responses.On the contrary, L-carnitine was found to reduce cholinergic responses in a great percentage of units and to inhibit L-acetylcarnitine-induced excitatory responses.Atropine blocked the increase in firing rate induced by L-carnitine and L-acetylcarnitine, thus suggesting for both of them a muscarinic activity.No interactions were observed between carnitines and GABA and glutamate.These results show that carnitine and acetylcarnitine are stereospecific neuroactive compounds with a cholinomimetic activity. They may play a role in a modulatory system for the cholinoceptive cortical neuron.