Kinking the double helix by bending deformation.
Kinking the double helix by bending deformation.
复制标题
通过弯曲变形来扭结双螺旋。
DOI：
10.1093/nar/gkm1125
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发表时间：
2008-03
影响因子：
14.9
通讯作者：
Vologodskii, Alexander
中科院分区：
文献类型：
--
作者：
Du, Quan;Kotlyar, Alexander;Vologodskii, Alexander
DNA在细胞内发挥功能时经常发生弯曲和扭转变形，这可能会导致常规螺旋结构的局部破坏。负扭转应力引起的破坏已被详细研究，而弯曲应力引起的破坏仅从理论上进行了分析。通过探测非常小的DNA环的结构，我们确定当DNA曲率半径小于3.5 nm时，弯曲应力破坏规则的螺旋结构。首先，我们开发了一种有效的方法来获得共价闭合的DNA微环。为了检测微环的结构破坏，我们用单链特异性核酸内切酶对其进行了处理。数据表明，在64-65-bp微环中，规则的DNA结构被弯曲变形破坏，但在85-86-bp微环中不被破坏。我们的研究结果表明，强大的DNA弯曲启动扭结形成，同时保持碱基配对。
DNA bending and torsional deformations that often occur during its functioning inside the cell can cause local disruptions of the regular helical structure. The disruptions created by negative torsional stress have been studied in detail, but those caused by bending stress have only been analyzed theoretically. By probing the structure of very small DNA circles, we determined that bending stress disrupts the regular helical structure when the radius of DNA curvature is smaller than 3.5 nm. First, we developed an efficient method to obtain covalently closed DNA minicircles. To detect structural disruptions in the minicircles we treated them by single-strand-specific endonucleases. The data showed that the regular DNA structure is disrupted by bending deformation in the 64–65-bp minicircles, but not in the 85–86-bp minicircles. Our results suggest that strong DNA bending initiates kink formation while preserving base pairing.
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DOI：
10.1073/pnas.68.4.815
发表时间：
1971-01-01
影响因子：
11.1
作者：
FULLER, FB
通讯作者：
FULLER, FB
影响因子：
5.6
作者：
Protozanova, E;Yakovchuk, P;Frank-Kamenetskii, MD
通讯作者：
Frank-Kamenetskii, MD
影响因子：
5.6
作者：
BENHAM, CJ
通讯作者：
BENHAM, CJ
影响因子：
2.9
作者：
LEBRET, M
通讯作者：
LEBRET, M
影响因子：
14.9
作者：
Lee, Joon-Hwa;Choi, Yun-Jeong;Choi, Byong-Seok
通讯作者：
Choi, Byong-Seok