Influences of polysaccharides in wood cell walls on lignification in vitro
Influences of polysaccharides in wood cell walls on lignification in vitro
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DOI：
10.1007/s10570-021-04108-x
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发表时间：
2021-07-30
期刊：
影响因子：
5.7
通讯作者：
Uraki, Yasumitsu
中科院分区：
文献类型：
--
作者：
Lyu, Yan;Matsumoto, Tatsuya;Uraki, Yasumitsu
为研究纤维素、水溶性木聚糖（WXY）、半乳葡甘露聚糖（GGM）和木葡聚糖（XG）等多糖对离体木质化的影响，以纤维素和半纤维素为原料制备人工多糖基质，并在此基质存在下，利用辣根过氧化物酶（HRP）催化松柏醇脱氢聚合物（DHP）的合成。在DHP形成之前，纤维素和半纤维素之间的相互作用进行了研究，半纤维素在细菌纤维素（BC）膜上的平衡吸附和石英晶体微天平耗散技术（QCM-D），以确定它们在纤维素纳米纤维（CNFs）上的吸附。结果表明，吸附量大小顺序为XG > GGM > WXY。QCM-D实验还表明HRP与纤维素而不是半纤维素强烈相互作用。在制备的基质中，XG-BC基质中产生的DHP的量是最大的，并且XG促进了5-5 '单元间键的形成。因此，XG一定参与了初生木材细胞壁的木质化。另一方面，GGM-BC基质中的DHP量最小，表明GGM阻碍了木质化。
To elucidate the effects of polysaccharides, cellulose, water-soluble xylan (WXY), galactoglucomannan (GGM) and xyloglucan (XG) on lignification in vitro, artificial polysaccharide matrices were prepared from a combination of cellulose and hemicelluloses, and dehydrogenation polymer (DHP) was synthesized from coniferyl alcohol in the presence of the matrices by using horseradish peroxidase (HRP). Prior to DHP formation, interactions between cellulose and hemicelluloses were investigated with equilibrium adsorptions of the hemicelluloses on bacterial cellulose (BC) films and with quartz crystal microbalance with dissipation technique (QCM-D) to determine their adsorption on cellulose nanofibers (CNFs). Both analyses showed that the order of adsorption amounts was XG > GGM > WXY. The QCM-D experiments also suggested that HRP strongly interacted with cellulose rather than hemicelluloses. The amount of DHP generated in the XG-BC matrix was the largest among the prepared matrices, and XG facilitated the formation of 5-5 ' interunitary linkages. Thus, XG must be involved in the lignification in primary wood cell wall. On the other hand, the amount of DHP in the GGM-BC matrix was the smallest, indicating that GGM hampered lignification.