1-ALPHA,25-DIHYDROXYVITAMIN-D3 PROMOTES FUSION OF MOUSE ALVEOLAR MACROPHAGES BOTH BY A DIRECT MECHANISM AND BY A SPLEEN CELL-MEDIATED INDIRECT MECHANISM
1-ALPHA,25-DIHYDROXYVITAMIN-D3 PROMOTES FUSION OF MOUSE ALVEOLAR MACROPHAGES BOTH BY A DIRECT MECHANISM AND BY A SPLEEN CELL-MEDIATED INDIRECT MECHANISM
复制标题
DOI：
10.1073/pnas.80.18.5583
复制
发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
SUDA, T
中科院分区：
文献类型：
--
作者：
ABE, E;MIYAURA, C;SUDA, T
小鼠肺泡巨噬细胞经15.mu处理后的条件培养基诱导广泛融合。g植物血凝素或豆豆蛋白A/ml或12 nM 1. α。，25-二羟基维生素D3 [1. α .,25(OH2)D3]。第3天融合率为80-90%。另外，1.alpha。直接添加到肺泡巨噬细胞中的25(OH)2D3在第3天诱导了约35%的细胞融合，而直接添加植物血凝素和豆豆蛋白A则完全没有促进融合。脾细胞或T细胞培养基经12 nM 1 α处理。1. α,25(OH)2D3应用于Sephadex G-100色谱柱，融合因子（MW 37,000-70,000）可从1. α,25(OH)2D3分离。1.α。在0.012-120 nM,25(OH)2D3可通过直接作用和脾细胞介导的间接作用诱导细胞融合，但在不同浓度的维生素下，后者的融合率都远高于前者。在测试的维生素D3衍生物中，1。25(OH)2D3最强，1. α次之。，24R，25-三羟基维生素D3, 1. α。-羟维生素D3, 25-羟维生素D3和24R，25-二羟维生素D31.α。，25(OH)2D3通过直接和间接两种机制诱导小鼠肺泡巨噬细胞融合，后者由脾细胞介导，可能由T细胞介导。
Extensive fusion was induced in mouse alveolar macrophages by treatment with conditioned media obtained from spleen cell cultures treated with 15 .mu.g of phytohemagglutinin or concanavalin A/ml or with 12 nM 1.alpha.,25-dihydroxyvitamin D3 [1.alpha.,25(OH2)D3]. The fusion rate was 80-90% on day 3. In addition, 1.alpha.,25(OH)2D3 added directly to alveolar macrophages induced fusion of about 35% of the cells on days 3, whereas direct addition of phytohemagglutinin and concanavalin A did not enhance fusion at all. When conditioned media from spleen cell or T cell cultures treated with 12 nM 1.alpha.,25(OH)2D3 were applied to a Sephadex G-100 column, a fusion factor (MW 37,000-70,000) could be separated from 1.alpha.,25(OH)2D3. 1.alpha.,25(OH)2D3 induced fusion at 0.012-120 nM in a dose-dependent manner both by direct action and by spleen cell-mediated indirect action, but the fusion rate was always much greater in the latter than in the former at each concentration of the vitamin. Of the vitamin D3 derivatives tested, 1.alpha.,25(OH)2D3 was the most potent, followed successively by 1.alpha.,24R,25-trihydroxyvitamin D3, 1.alpha.-hydroxyvitamin D3, 25-hydroxyvitamin D3 and 24R,25-dihydroxyvitamin D3. 1.alpha.,25(OH)2D3 induces fusion of mouse alveolar macrophages by both a direct and an indirect mechanism, the latter mediated by spleen cells, probably by T cells.