Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza a and influenza B viruses and respiratory syncytial viruses in children
Evaluation of the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for detection of influenza a and influenza B viruses and respiratory syncytial viruses in children
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DOI：
10.1128/jcm.00959-07
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发表时间：
2008-02-01
影响因子：
9.4
通讯作者：
Lebon, Pierre
中科院分区：
文献类型：
--
作者：
LeGoff, Jerome;Kara, Rachid;Lebon, Pierre
We evaluated the one-step multiplex real-time reverse transcription-PCR ProFlu-1 assay for the detection of influenza A and influenza B viruses and respiratory syncytial viruses from 353 pediatric nasopharyngeal aspirates. As assessed by comparison with the results of immunofluorescence testing and cell culture, the specificity and the sensitivity of the ProFlu-1 assay ranged from 97% to 100%. In addition, the ProFlu-1 assay amplified 9% of samples not detected by conventional methods.
