Isolation and characterisation of the lipopolysaccharide from Acidiphilium strain GS18h/ATCC55963, a soil isolate of Indian copper mine
Isolation and characterisation of the lipopolysaccharide from Acidiphilium strain GS18h/ATCC55963, a soil isolate of Indian copper mine
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DOI：
10.1016/j.femsle.2005.04.001
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发表时间：
2005-05-15
影响因子：
2.1
通讯作者：
Bhadra, R
中科院分区：
文献类型：
--
作者：
Bera, R;Nayak, A;Bhadra, R
革兰氏阴性嗜酸菌菌株GS 18 h/ATCC 55963的脂多糖（LPS）表现出非常低的内毒素活性，如通过鲎凝胶活性、半乳糖胺（GaIN）致敏小鼠的致死毒性和BALB/c小鼠血清中肿瘤坏死因子α（TNF α）水平所确定的。LPS的分析，特别是通常导致毒性的脂质A的分析，显示后者除了脂肪酸外还含有葡糖胺和磷酸盐，其中14：0（3-OH）、18：0（3-OH）、18：1和19：0（环）是主要组分，而12：0、16：0、19：1、20：0（3-OH）和20：1（3-OH）以少量存在。14：0（3-OH）和18：0（3-OH）脂肪酸是酰胺连接的，而其余的是酯键。该LPS的多糖部分含有葡萄糖、半乳糖、甘露糖、鼠李糖、庚糖、半乳糖醛酸和3-脱氧-D-甘露-辛-2-酮糖酸（Kdo）。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）的LPS显示大分子异质性明显不同于大肠杆菌或沙门氏菌。这种LPS的毒性极低，归因于其脂质A的脂肪酸组成，有望实现潜在的治疗应用。(c)2005年，欧洲微生物学会联合会。Elsevier B. V.出版，保留所有权利。
The lipopolysaccharide (LPS) of the Gram-negative Acidiphilium strain GS18h/ATCC55963, a new soil isolate, exhibited very low endotoxic activity as determined by Limulus gelation activity, lethal toxicity in galactosamine (GaIN) sensitised mice, and level of tumor necrosis factor alpha (TNF alpha) in the blood serum of BALB/c mice. Analysis of the LPS, specially of lipid A which usually accounts for the toxicity, revealed the latter to contain glucosamine and phosphate besides fatty acids, of which 14:0(3-OH), 18:0(3-OH), 18:1 and 19:0(cyclo) are the major components, while 12:0, 16:0, 19:1, 20:0(3-OH) and 20:1(3-OH) are present in small amounts. The 14:0(3-OH) and 18:0(3-OH) fatty acids are amide-linked, whereas the rest are ester bound. Glucose, galactose, mannose, rhamnose, heptose, galacturonic acid and 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) were present in the polysaccharide part of this LPS. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of the LPS showed a macromolecular heterogeneity distinctly different from those of Escherichia coli or Salmonella. The toxicity of this LPS being extremely low attributed to fatty acid composition of its lipid A, promises potential therapeutic application. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.