Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g)
Three kinds of extracellular glucosyltransferases from Streptococcus mutans 6715 (serotype g)
复制标题
变形链球菌6715（血清型g）的三种胞外葡萄糖基转移酶
DOI：
10.1016/0014-5793(83)81120-x
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发表时间：
1983
期刊：
影响因子：
3.5
通讯作者：
H. Mukasa
中科院分区：
文献类型：
--
作者：
A. Shimamura;H. Tsumori;H. Mukasa
除了1，3-α-D-葡聚糖合成酶（pI 4.9）和高度分支的1，6-α-D-葡聚糖合成酶（pI 3.9-4.1），变异链球菌6715（β-葡糖基PEG）以多种形式分泌第三种葡糖基转移酶（pI 5.5-7.0），其1，6-α-键形成活性为87%，1，3-α-键形成活性为6%，1，3，6-支链形成活性为7%。这种酶的生产是非常增强时，该生物体生长在吐温80补充培养基。这3种葡萄糖基转移酶均来自同一种微生物，它们在酶学和免疫学上各不相同，并且在大肠杆菌中普遍存在。
In addition to the 1,3-α-D-glucan synthetase (pI 4.9) and the highly-branched 1,6-α-D-glucan synthetase (pI 3.9–4.1),Streptococcus mutans6715 (serotypeg) was found to secrete the third glucosyltransferase in multiple forms (pI 5.5–7.0), which exhibited 87% 1,6-α-bond-, 6% 1,3-α-bond- and 7% 1,3,6-branch-forming activities. The production of this enzyme was extremely enhanced when the organism was grown in Tween 80-supplemented medium. The 3 glucosyltransferases from the same organism were enzymatically and immunologically distinct from each other, and they were commonly found among the serotypegstrains.