Persistent productive Epstein-Barr virus replication in normal epithelial cells in vivo.
Persistent productive Epstein-Barr virus replication in normal epithelial cells in vivo.
复制标题
体内正常上皮细胞中持续高效的 Epstein-Barr 病毒复制。
DOI：
10.1086/323992
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Adler-Storthz,K
中科院分区：
文献类型：
--
作者：
Walling,DM;Flaitz,CM;Nichols,CM;Hudnall,SD;Adler-Storthz,K
生产性EB病毒（EBV）的复制特征毛白斑，口腔上皮病变，通常发生在感染人类免疫缺陷病毒（HIV）的个人。在伐昔洛韦治疗前、治疗中和治疗后，从HIV感染者中获得了一系列舌活检标本。用Southern杂交检测EBV基因组末端线性片段，逆转录聚合酶链反应扩增EBV复制基因转录本，免疫组化检测EBV复制蛋白，原位杂交检测EBV DNA。在毛状白斑和正常舌组织中均检测到EBV复制。伐昔洛韦治疗完全废除了EBV在体内的复制，导致在决议的毛状白斑时，它是存在的。伐昔洛韦治疗后，正常舌上皮细胞中EBV复制恢复。这些数据表明，正常的口腔上皮支持HIV感染者持续的EBV感染，EBV的复制是必要的，但不足以导致口腔毛状白斑的发病
Productive Epstein-Barr virus (EBV) replication characterizes hairy leukoplakia, an oral epithelial lesion typically occurring in individuals infected with human immunodeficiency virus (HIV). Serial tongue biopsy specimens were obtained from HIV-infected subjects before, during, and after valacyclovir treatment. EBV replication was detected by Southern hybridization to linear terminal EBV genome fragments, reverse-transcriptase polymerase chain reaction amplification of EBV replicative gene transcripts, immunohistochemical detection of EBV replicative protein, and in situ hybridization to EBV DNA. EBV replication was detected in both hairy leukoplakia and normal tongue tissues. Valacyclovir treatment completely abrogated EBV replication in vivo, resulting in resolution of hairy leukoplakia when it was present. EBV replication returned in normal tongue epithelial cells after valacyclovir treatment. These data suggest that normal oral epithelium supports persistent EBV infection in individuals infected with HIV and that productive EBV replication is necessary but not sufficient for the pathogenesis of oral hairy leukoplakia