Serum Brain-Derived Neurotrophic Factor is Related to Platelet Reactivity but not to Genetic Polymorphisms within BDNF Encoding Gene in Patients with Type 2 Diabetes
Serum Brain-Derived Neurotrophic Factor is Related to Platelet Reactivity but not to Genetic Polymorphisms within BDNF Encoding Gene in Patients with Type 2 Diabetes
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DOI：
10.12659/msm.895607
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发表时间：
2016-01-07
影响因子：
3.1
通讯作者：
Postula, Marek
中科院分区：
文献类型：
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作者：
Eyileten, Ceren;Zaremba, Malgorzata;Postula, Marek
背景资料：本研究的目的是探讨脑源性神经营养因子（BDNF），血小板反应性和炎症标志物的血清浓度之间的关联，以及其与BDNF编码基因变异体在2型糖尿病患者（T2 DM）在乙酰水杨酸（阿萨）therapeutic.Material/Methods：这一回顾性，开放标签的研究纳入91例患者。检测血清BDNF、基因型变异、血液学、生化和炎症标志物。在阿萨末次给药后2-3小时的早晨采集血样。使用iPLEX Sequenom分析法分析选定变体的脑源性神经营养因子基因型。结果：在多元线性回归分析中，CADP-CT >74秒（p74秒（p=0.02）和IL-6浓度（p=0.03）是血清脑源性神经营养因子高于中位数的危险因素。内含子SNP rs 925946、错义SNP rs6265和内含子SNP rs 4923463等位基因组与研究队列中BDNF浓度之间观察到无显著差异。慢性炎症和增强的免疫系统与BDNF的产生有关，这可能是本研究中血小板反应性高的T2 DM患者血清BDNF水平高于血小板反应性正常的受试者的原因。
Background: The aim of this study was to investigate the association between serum concentrations of the brain-derived neurotrophic factor (BDNF), platelet reactivity and inflammatory markers, as well as its association with BDNF encoding gene variants in type 2 diabetic patients (T2DM) during acetylsalicylic acid (ASA) therapy.Material/Methods: This retrospective, open-label study enrolled 91 patients. Serum BDNF, genotype variants, hematological, biochemical, and inflammatory markers were measured. Blood samples were taken in the morning 2-3 h after the last ASA dose. The BDNF genotypes for selected variants were analyzed by use of the iPLEX Sequenom assay.Results: In multivariate linear regression analysis, CADP-CT >74 sec (p74 sec (p=0.02) and IL-6 concentration (p=0.03) were risk factors for serum BDNF above the median. Non-significant differences were observed between intronic SNP rs925946, missense SNP rs6265, and intronic SNP rs4923463 allelic groups and BDNF concentrations in the investigated cohort.Conclusions: Chronic inflammatory condition and enhanced immune system are associated with the production of BDNF, which may be why the serum BDNF level in T2DM patients with high platelet reactivity was higher compared to subjects with normal platelet reactivity in this study.