The effects of 3-methylcholanthrene on macrophage respiratory burst and biotransformation activities in the common carp (Cyprinus carpio L.).
The effects of 3-methylcholanthrene on macrophage respiratory burst and biotransformation activities in the common carp (Cyprinus carpio L.).
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3-甲基胆蒽对鲤鱼（Cyprinus carpio L.）巨噬细胞呼吸爆发和生物转化活性的影响。
DOI：
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发表时间：
2002
影响因子：
4.7
通讯作者：
P. Deschaux
中科院分区：
文献类型：
--
作者：
S. Reynaud;D. Marionnet;L. Taysse;C. Duchiron;P. Deschaux
以鲤鱼（Cyprinus carpio L.）为对象，研究了吞噬细胞功能作为多环芳烃污染胁迫生物指示剂的敏感性。在腹腔注射原型Cyp 1A诱导剂（3-甲基胆蒽）后1、2、3、5和7天测量头肾巨噬细胞呼吸爆发的时程反应。将该免疫活性与肝脏和头肾中总细胞色素P450、乙氧基试卤灵O-脱乙基酶活性（EROD）和谷胱甘肽S-转移酶活性（GST）的诱导率进行比较。3-甲基胆蒽（40 mg kg（-1））引起巨噬细胞呼吸爆发的迅速增加。这种反应在暴露后第3天达到最大，与肝脏和头肾中细胞色素P450和EROD活性的最大诱导相一致。此外，α-萘黄酮，其功能作为Ah受体拮抗剂和细胞色素P450 1A活性的抑制剂，逆转了3-甲基胆蒽诱导的免疫和酶参数测量，这表明代谢过程。综上所述，这些结果表明，巨噬细胞氧化功能的诱导可能是一个同样敏感的标记暴露于多环芳烃作为诱导的生物转化活动，并确认由Ah受体介导的反应是类似的，如果不是相同的，那些哺乳动物。
The sensitivity of phagocytic cell function as a bioindicator of pollution stress by polycyclic aromatic hydrocarbons was evaluated in the common carp (Cyprinus carpio L). The time course response of the head-kidney macrophage respiratory burst was measured 1, 2, 3, 5 and 7 days after intraperitoneal injection of a prototypical Cyp 1A inducer (3-methylcholanthrene). This immune activity was compared to the rate of induction of total cytochrome P450, ethoxyresorufin O-deethylase activity (EROD) and glutathione S-transferase activity (GST) in the liver and head-kidney. 3-methylcholanthrene (40 mg kg(-1)) caused a rapid increase in the macrophage respiratory burst. This response was maximal at day 3 post exposure and coincided with maximum induction of cytochrome P450 and EROD activity in liver and head-kidney. Moreover, alpha-naphtoflavone, which functions as both an Ah receptor antagonist and an inhibitor of cytochrome P450 1A activity, reversed the 3-methylcholanthrene induction of immune and enzymatic parameters measured, suggesting metabolic processes. Taken together these results suggest that the induction of macrophage oxidative function may be an equally sensitive marker of exposure to polycyclic aromatic hydrocarbon as the induction of biotransformation activities and confirm that responses mediated by the Ah receptor are similar, if not identical, to those of mammals.
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体内反复暴露于苯并[a]芘后，评估苯并[a]芘的小鼠脾细胞类型代谢和体外功能。
影响因子：
3.8
作者：
Ladics,GS;Kawabata,TT;Munson,AE;WhiteJr,KL
通讯作者：
WhiteJr,KL
α-萘黄酮作为 2,3,7,8-四氯二苯并-对二恶英诱导的 CYP1A1 基因表达抑制剂的作用机制。
影响因子：
3.9
作者：
Merchant,M;Arellano,L;Safe,S
通讯作者：
Safe,S
影响因子：
7.4
作者：
Puntarulo, S;Cederbaum, AI
通讯作者：
Cederbaum, AI
小鼠脾细胞类型对苯并[a]芘的代谢。
影响因子：
3.8
作者：
Ladics,GS;Kawabata,TT;Munson,AE;WhiteJr,KL
通讯作者：
WhiteJr,KL
苯并（a）芘代谢物抑制小鼠脾细胞的体外体液免疫反应，以及α-萘黄酮抑制苯并（a）芘诱导的免疫抑制。
影响因子：
11.2
作者：
Kawabata,TT;WhiteJr,KL
通讯作者：
WhiteJr,KL