Genetic analysis of the reaction level of self-incompatibility to a 4% CO2 gas treatment in the radish (Raphanus sativus L.).
Genetic analysis of the reaction level of self-incompatibility to a 4% CO2 gas treatment in the radish (Raphanus sativus L.).
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DOI：
10.1007/s001220051596
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发表时间：
2000-12-01
影响因子：
5.4
通讯作者：
Matsuura, S
中科院分区：
文献类型：
--
作者：
Niikura, S;Matsuura, S
萝卜的自交不亲和性（SI）是由一系列复等位基因组成的S基因座决定的。这种SI可以通过CO2气体处理来克服，这是一种在获得大量F-1商业种子的亲本种子中非常有用的特性。我们从经验中知道，萝卜对4%CO2气体处理的自交不亲和反应水平（RLSI）存在遗传变异，此后描述为RLSICO 2）。本研究以9324（S-206纯合子，低RLSICO_2）与LV 364（S-209纯合子，高RLSICO_2）杂交的F_1代为材料，培育了一个F_2代群体，并对其进行了分析。S-206纯合子、（SS-209）-S-206杂合子、S-209纯合子的RLSICO 2在F-2群体中均符合单基因分离的理论比例，但差异不显著。因此，我们得出结论，RLSICO 2，是由一个基因以外的S-基因控制。在该F-2群体中，RLSICO 2的分离符合3（低RLSICO 2）：1（高RLSICO 2）的比例。这一结果和F-3后代测定表明，高RLSICO_2是由隐性基因控制的。具有不同RLSICO 2的S-209-纯合子之间的互交表明，该基因将在柱头中起作用。
In radishes, self-incompatibility (SI) is governed by the S-locus, which consists of a series of multiple alleles. This SI can be overcome by CO2 gas treatment, a characteristic that is very useful in obtaining large amounts of parental seeds for F-1 commercial seeds. We know from experience that there are genetic variations in the reaction level of self-incompatibility (RLSI) to a 4% CO2 gas treatment thereafter described as RLSICO2) in the radish. We have raised and analyzed an F-2 population derived from an F-1 cross between No. 9324 (S-206-homozygote, low RLSICO2) and LV364 (S-209-homozygote, high RLSICO2). The RLSICO2 among three S-genotypes (S-206-homozygotes, (SS209)-S-206 heterozygotes, S-209-homozygotes), which fit the theoretical ratio of one gene segregation in the F-2 population, did not show any significant statistical differences. Hence, we concluded that the RLSICO2, was controlled by a gene other than the S-gene. In this F-2 population the segregation of the RLSICO2, fit the 3(low RLSICO2):1(high RLSICO2) ratio well. This result and F-3 progeny tests suggest that high RLSICO2 is controlled by a recessive gene. Reciprocal crosses among S-209-homozygotes with different RLSICO2 have shown that this gene would act in the stigma.