A bioluminescent enzyme immunoassay for prostaglandin E(2) using Cypridina luciferase.
A bioluminescent enzyme immunoassay for prostaglandin E(2) using Cypridina luciferase.
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DOI：
10.1002/bio.1086
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发表时间：
2009-03
期刊：
影响因子：
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通讯作者：
Chun Wu;Saki Irie;Shozo Yamamoto;Y. Ohmiya
中科院分区：
文献类型：
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作者：
Chun Wu;Saki Irie;Shozo Yamamoto;Y. Ohmiya
前列腺素E（2）是花生四烯酸的主要环氧合酶代谢产物之一。我们开发了一种利用生物发光酶Cypridina荧光素酶的前列腺素E（2）竞争性免疫吸附测定法。前列腺素E（2）的量可以在7.8至500 pg/mL的浓度范围内定量。置换50%最大结合率的海萤属海藻酶标记的前列腺素E（2）（B/B（0））所需的未标记前列腺素E（2）的量约为35 pg/mL。结果表明，海萤虫荧光素酶作为一种新型的酶联免疫吸附分析标记酶具有很大的潜力。
Prostaglandin E(2) is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E(2) utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E(2) amount could be quantified over the concentration ranging from 7.8 to 500 pg/mL. The amount of unlabeled prostaglandin E(2) required to displace 50% of the maximal binding of Cypridina luciferase-labeled prostaglandin E(2) (B/B(0)) was approximately 35 pg/mL. The results show a great potential of Cypridina luciferase as a new labeling enzyme for enzyme-linked immunosorbent assay.