Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression
Genistein, daidzein, and resveratrols stimulate PGC-1β-mediated gene expression
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金雀异黄素、大豆黄酮和白藜芦醇刺激 PGC-1β 介导的基因表达
DOI：
10.1016/j.bbrep.2018.11.009
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发表时间：
2019
影响因子：
2.7
通讯作者：
Kamei Yasutomi
中科院分区：
文献类型：
--
作者：
Uchitomi Ran;Nakai Shiho;Matsuda Rintaro;Onishi Takumi;Miura Shinji;Hatazawa Yukino;Kamei Yasutomi
PGC-1β是核受体如雌激素受体相关受体（ERR）的转录共激活因子。小鼠中PGC-1β的转基因过表达增加能量消耗并抑制高脂饮食诱导的肥胖在这项研究中，我们使用报告基因分析系统来检测PGC-1β的转录活性，以筛选各种食物源性和天然化合物。α-羟异黄酮、染料木素和大豆苷元以及几种白藜芦醇激活PGC-1β。在PGC-1β存在的情况下，染料木黄酮、大豆苷元和反式-氧化白藜芦醇激活了ERR反应元件介导的报告活性。C2 C12成肌细胞中PGC-1β的稳定过表达增加了脂肪酸β-氧化的重要酶中链酰基辅酶A脱氢酶（MCAD）的表达。Genistein和黄豆苷元可增加表达PGC-1β的C2 C12细胞MCADmRNA水平和线粒体含量。这些化合物激活ERR/PGC-1β复合物介导的基因表达，我们的研究结果可能为开发针对肥胖的功能性食品提供实用基础。
PGC-1β is a transcriptional co-activator of nuclear receptors such as the estrogen receptor-related receptor (ERR). Transgenic overexpression of PGC-1β in mice increases energy expenditure and suppresses high-fat diet-induced obesity. In this study, we screened various food-derived and natural compounds using a reporter assay system to measure the transcriptional activity of PGC-1β. Soy-derived isoflavones, genistein and daidzein, and several resveratrols activated PGC-1β. Genistein, daidzein, and trans-oxyresveratrol activated ERR-responsive element-mediated reporter activity in the presence of PGC-1β. Stable overexpression of PGC-1β in C2C12 myoblasts increased the expression of medium-chain acyl-CoA dehydrogenase (MCAD), an important enzyme in fatty acid β-oxidation. Genistein and daidzein increasedMCADmRNA levels and mitochondrial content in PGC-1β-expressing C2C12 cells. These compounds activated ERR/PGC-1β complex-mediated gene expression, and our findings may be a practical foundation for developing functional foods targeting obesity.