Temporal profile of vascular changes induced by systemic nitroglycerin in the meningeal and cortical districts
Temporal profile of vascular changes induced by systemic nitroglycerin in the meningeal and cortical districts
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DOI：
10.1177/0333102410379887
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发表时间：
2011-01-01
期刊：
影响因子：
4.9
通讯作者：
Tassorelli, Cristina
中科院分区：
文献类型：
--
作者：
Greco, Rosaria;Meazza, Cristina;Tassorelli, Cristina
背景资料：临床研究表明，一氧化氮（NO）供体引起脑血流量（CBF）的局部变化，类似于自发性偏头痛的报告。全身性硝酸甘油（NTG）是一种NO供体，是公认的偏头痛实验模型。在这项研究中，我们已经研究了NTG对脑膜和皮质血流量rats.Methods的影响：区域血流量监测在雄性Sprague-Dawley大鼠使用激光多普勒血流仪之前和之后NTG/生理盐水注射超过150分钟。预处理N ω-硝基-L-精氨酸酯（L-NAME）或7-硝基吲唑（7-NI）对NTG诱导的变化对血流的影响也investigated.Results：在硬膜NTG引起的双相反应所代表的最初的血流量减少，随后显着增加。在差异，在皮质NTG只造成血流量的增加。预处理无论是L-NAME或7-NI防止NTG诱导的血流量增加，在这两个地区，而只有L-NAME也防止NTG诱导的硬膜血流量减少。结论：目前的研究结果提供了额外的信息NTG对血流量的影响在脑膜和皮质水平的时间。这些作用似乎与血管调节机制和/或响应内源性NO合成的代谢活性有关。
Background: Clinical studies indicated that nitric oxide (NO) donors cause regional changes in cerebral blood flow (CBF), similar to those reported in spontaneous migraine. Systemic nitroglycerin (NTG), a NO donor, is a well-accepted experimental model of migraine. In this study we have examined the effects of NTG on the meningeal and cortical blood flow in rats.Methods: Regional blood flow was monitored in male Sprague-Dawley rats using laser Doppler flowmetry before and after NTG/saline injection over 150 minutes. The effect of pre-treatment with N omega-nitro-L-arginine ester (L-NAME) or 7-nitroindazole (7-NI) on NTG-induced changes on blood flow was also investigated.Results: In the dura NTG caused a biphasic response represented by an initial decrease in blood flow followed by a significant increase. At variance, in the cortex NTG caused only an increase in blood flow. Pre-treatment with either L-NAME or 7-NI prevented NTG-induced increase in blood flow in both districts, while only L-NAME also prevented NTG-induced decrease in dural blood flow.Conclusion: The present findings provide additional information on the timing of effects of NTG on blood flow at both the meningeal and cortical levels. These effects seem to be related to vasoregulatory mechanisms and/or metabolic activity in response to the synthesis of endogenous NO.