Visual cortex damage in a ferret model of ocular hypertension
Visual cortex damage in a ferret model of ocular hypertension
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DOI：
10.1007/s10384-022-00901-8
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发表时间：
2022-01-19
影响因子：
2.4
通讯作者：
Aihara, Makoto
中科院分区：
文献类型：
--
作者：
Fujishiro, Takashi;Honjo, Megumi;Aihara, Makoto
目的应用细胞色素氧化酶（CO）染色分析高眼压雪貂模型视觉皮层的变化。研究设计：实验性。方法将培养的结膜细胞注入右眼前房，对9只雪貂进行OH诱导。3只雪貂作为对照。CO染色评估视觉皮层II-III层和IVC层的代谢强度。结果OH雪貂初级视觉皮层（V1）右侧和左侧II-III层CO染色强度分别为39.8 +/- 10.3和41.9 +/- 9.2任意单位。对照雪貂的强度为88.1±8.1任意单位。OH眼II-III层的CO染色强度显著低于对照眼（unpaired t检验，P < 0.01）。OH雪貂V1左、右IVC层CO染色强度分别为60.3 +/- 12.8和60.0 +/- 13.5任意单位。在对照雪貂中，强度为111.4±9.6任意单位。OH眼IVC层CO染色强度显著低于对照眼（未配对t检验，P < 0.01）。结论OH眼视皮层CO染色强度降低。这项研究表明，OH引起视觉皮层的代谢变化。
Purpose We aimed to analyze the changes in the visual cortex of a ferret model of ocular hypertension (OH) using cytochrome oxidase (CO) staining. Study design Experimental. Methods OH was induced in 9 ferrets by means of injection of cultured conjunctival cells into the anterior chamber of the right eye. Three ferrets were used as the controls. CO staining was performed to assess the metabolic intensity at the II-III and IVC layers of the visual cortex. Results The intensities of CO staining in the right and left II-III layers of the primary visual cortex (V1) in the OH ferrets were 39.8 +/- 10.3 and 41.9 +/- 9.2 arbitrary units, respectively. In the control ferrets, the intensity was 88.1 +/- 8.1 arbitrary units. The intensity of CO staining of the II-III layers obtained from the OH eyes was significantly lower than that from the control eyes (unpaired t test, P < .01). The intensities of CO staining in the right and left IVC layers of V1 in the OH ferrets were 60.3 +/- 12.8 and 60.0 +/- 13.5 arbitrary units, respectively. In the control ferrets, the intensity was 111.4 +/- 9.6 arbitrary units. The CO staining intensity of the IVC layer obtained from the OH eyes was significantly lower than that from the control eyes (unpaired t test, P < .01). Conclusion The CO staining intensity was reduced in the visual cortex from OH eyes. This study revealed that OH causes metabolic change in the visual cortex.