Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis.
Tudor Domain Containing Protein TDRD12 Expresses at the Acrosome of Spermatids in Mouse Testis.
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DOI：
10.5713/ajas.15.0436
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发表时间：
2016-07
期刊：
影响因子：
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通讯作者：
Choi Y
中科院分区：
文献类型：
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作者：
Kim M;Ki BS;Hong K;Park SP;Ko JJ;Choi Y
Tdrd12是Tdrd家族成员之一。然而，Tdrd12的表达模式尚未得到很好的研究。为了比较Tdrd 12在各种组织中的表达水平，使用来自肝脏、小肠、心脏、脑、肾、肺、脾、胃、子宫、卵巢和睾丸的总RNA进行真实的时间聚合酶链反应。Tdrd 12 mRNA在睾丸中高表达。利用小鼠TDRD 12的氨基酸残基SQRPNEKPLRLTEKKDC制备抗小鼠TDRD 12的抗体，以研究TDRD 12在睾丸中的定位。免疫组化显示TDRD 12主要定位于成年睾丸曲细精管中的精子细胞。在出生后发育期间，TDRD 12差异表达。2周时，TDRD 12在早期精母细胞中被检测到，TDRD 12定位于圆形精子细胞的顶体。TDRD12表达不与TDRD1共定位，TDRD1是生殖细胞中皮尔纳途径的重要组分。我们的研究结果表明，TDRD 12可能在精子细胞中发挥重要作用，并依赖TDRD 1作为精子发生的调节因子。
Tdrd12 is one of tudor domain containing (Tdrd) family members. However, the expression pattern of Tdrd12 has not been well studied. To compare the expression levels of Tdrd12 in various tissues, real time-polymerase chain reaction was performed using total RNAs from liver, small intestine, heart, brain, kidney, lung, spleen, stomach, uterus, ovary, and testis. Tdrd12 mRNA was highly expressed in testis. Antibody against mouse TDRD12 were generated using amino acid residues SQRPNEKPLRLTEKKDC of TDRD12 to investigate TDRD12 localization in testis. Immunostaining assay shows that TDRD12 is mainly localized at the spermatid in the seminiferous tubules of adult testes. During postnatal development, TDRD12 is differentially expressed. TDRD12 was detected in early spermatocytes at 2 weeks and TDRD12 was localized at acrosome of the round spermatids. TDRD12 expression was not co-localized with TDRD1 which is an important component of piRNA pathway in germ cells. Our results indicate that TDRD12 may play an important role in spermatids and function as a regulator of spermatogenesis in dependent of TDRD1.