DIFFERENCE BETWEEN RANDOM MOVEMENT AND CHEMOTAXIS - EFFECTS OF ANTITUBULINS ON NEUTROPHIL GRANULOCYTE LOCOMOTION
DIFFERENCE BETWEEN RANDOM MOVEMENT AND CHEMOTAXIS - EFFECTS OF ANTITUBULINS ON NEUTROPHIL GRANULOCYTE LOCOMOTION
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DOI：
10.1016/0014-4827(74)90618-1
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发表时间：
1974-01-01
影响因子：
3.7
通讯作者：
NORBERG, B
中科院分区：
文献类型：
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作者：
BANDMANN, U;RYDGREN, L;NORBERG, B
抗微管蛋白demecolcine和鬼臼酰肼（SPI）被用于实验设计，以阐明中心粒相关的细胞质微管在人中性粒细胞（PMN）的运动中的作用。中性粒细胞的运动被研究为趋化性和随机运动的速度。地美秋胺（0.01 μg/ml）和SPI（0.1 μg/ml）均抑制PMN的趋化性，即浓度低于报道的有丝分裂中期阻滞阈值。0.5 μg/ml SPI处理后，单个PMN在随机运动过程中的速度仅略有降低。SPI（0.5 μg/ml）对PMN运动无明显抑制作用。上述差异表明，中心粒相关微管是中性粒细胞趋化性定向运动或中性粒细胞定向运动的重要结构，但不是中性粒细胞运动机制的重要结构。目前的观察结果可能，至少部分地，解释了抗微管蛋白对急性痛风的有益作用。
The antitubulins demecolcine and podophyllic acid ethylhydrazide (SPI) were used in experiments designed to elucidate the role of centriole-associated cytoplasmic microtubules in the locomotion of human neutrophil granulocytes (PMNs). The PMN locomotion was studied as chemotaxis and as the velocity of random movement. The PMN chemotaxis was inhibited by demecolcine (0.01 μg/ml) and SPI (0.1 μg/ml), i.e. concentrations below the reported threshold ones for mitotic arrest in metaphase. The velocity of single PMNs during random movement was only slightly reduced by treatment with 0.5 μg/ml of SPI. PMN locomotion was not appreciably inhibited by SPI, 0.5 μg/ml. The discrepancies mentioned suggest that centriole-associated microtubules are essential structures in the PMN direction-finding or PMN directional movement of chemotaxis but not in the mechanism of PMN locomotion. The present observations might, at least in part, explain the beneficial effects of antitubulins on acute gout.