Dual-Mode Ultrasensitive Quantification of MicroRNA in Living Cells by Chiroplasmonic Nanopyramids Self-Assembled from Gold and Upconversion Nanoparticles
Dual-Mode Ultrasensitive Quantification of MicroRNA in Living Cells by Chiroplasmonic Nanopyramids Self-Assembled from Gold and Upconversion Nanoparticles
复制标题
利用金和上转换纳米粒子自组装的手性纳米金字塔对活细胞中的 MicroRNA 进行双模式超灵敏定量
DOI：
10.1021/jacs.5b10309
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发表时间：
2016-01-13
影响因子：
15
通讯作者：
Xu, Chuanlai
中科院分区：
文献类型：
--
作者：
Li, Si;Xu, Liguang;Xu, Chuanlai
具有生物应用的手性自组装纳米材料引起了人们的极大兴趣。在本研究中，构建了DNA驱动的金上转换纳米颗粒(Au-UCNP)金字塔，用于实时检测细胞内microRNA(MiRNA)。Au-UCNP金字塔具有双光学活性，在521 nm处表现出较强的等离子体圆二色谱(CD)，在500-600 nm处表现出明显的发光，因此可以用它们来监测。在miRNA的存在下，Cd降低，发光强度增加。实验结果表明，镉的发光强度在0.073~43.65fmo1/10ug(核糖核酸)范围内呈良好的线性关系，检出限为0.03fmo1/10ug(核糖核酸)；发光强度在0.16~43.65fmo1/10mgRNA之间，检出限为0.12fmo1/10mgRNA。这些数据表明CD信号对miRNA的浓度比发光信号更敏感，这归因于由于光子与手性纳米结构相互作用的自旋角动量以及金字塔中DNA分子本征手性的等离子体增强而产生的强烈的CD强度。这种方法为超灵敏检测和定量活细胞中的miRNA开辟了一条新的途径。
Chiral self-assembled nanomaterials with biological applications have attracted great interest. In this study, DNA-driven gold-upconversion nanoparticle (Au-UCNP) pyramids were fabricated to detect intracellular microRNA (miRNA) in real time. The Au-UCNP pyramids are doubly optically active, displaying strong plasmonic circular dichroism (CD) at 521 nm and significant luminescence in 500-600 nm, and therefore can be monitored by both of them. CD will decrease while the luminescence intensity increases in the presence of miRNA. The experimental results show that the CD intensity had an outstanding linear range from 0.073 to 43.65 fmol/10 mu g(RNA) and a limit of detection (LOD) of 0.03 fmol/10 mu g(RNA), whereas the luminescence intensity ranged from 0.16 to 43.65 fmol/10 mu g(RNA) with a LOD of 0.12 fmo1/10 mu g(RNA). These data indicate that the CD signal is much more sensitive to the concentration of miRNA than the luminescent signal, which is attributed to the strong CD intensity arising from the spin angular momentum of the photon interaction with chiral nanostructures and the plasmonic enhancement of the intrinsic chirality of DNA molecules in the pyramids. This approach opens up a new avenue to the ultrasensitive detection and quantification of miRNA in living cells.