FLUORESCENCE QUENCHING METHOD FOR DETERMINING EQUILIBRIUM-CONSTANTS FOR POLYCYCLIC AROMATIC-HYDROCARBONS BINDING TO DISSOLVED HUMIC MATERIALS
FLUORESCENCE QUENCHING METHOD FOR DETERMINING EQUILIBRIUM-CONSTANTS FOR POLYCYCLIC AROMATIC-HYDROCARBONS BINDING TO DISSOLVED HUMIC MATERIALS
复制标题
DOI：
10.1021/es00153a012
复制
发表时间：
1986-11-01
影响因子：
11.4
通讯作者：
GRANT, CL
中科院分区：
文献类型：
--
作者：
GAUTHIER, TD;SHANE, EC;GRANT, CL
建立了一种测定芘、菲和蒽与溶解态腐殖酸和富里酸缔合平衡常数的荧光猝灭法。该技术是基于多环芳烃（PAH）在水溶液中的荧光淬灭后，与腐殖物质的观察。缔合常数来自于荧光强度的分数减少作为添加腐殖物质的函数与Stern-Volmer图。这允许在不分离的情况下测量缔合常数。此外，该技术涉及比率测量，因此不需要确定污染物的确切浓度。Stern-Volmer图的线性为三个多环芳烃的研究。蒽腐殖酸缔合常数的荧光猝灭技术确定的相关性相当好，通过反相方法确定的值。然而，与反相法不同，测定的缔合常数不依赖于溶解有机物的浓度。
A fluorescence quenching method has been developed for determining equilibrium constants for the association of pyrene, phenanthrene, and anthracene with dissolved humic and fulvic acids. The technique is based upon the observation that polycyclic aromatic hydrocarbon (PAH) fluorescence in aqueous solution is quenched upon association with humic material. Association constants are derived from the fractional decrease in fluorescence intensity as a function of added humic material with Stern-Volmer plots. This allows association constants to be measured without a separation. In addition, the technique involves a ratio measurement so the exact concentration of pollutant need not be determined. Stern-Volmer plots were linear for the three PAHs studied. Anthracene-humic association constants determined by the fluorescence quenching technique correlated reasonably well with values determined by a reverse-phase method. However, unlike the reverse-phase method the measured association constants do not depend on the concentration of dissolved organic matter.