Visualizing stimulus convergence in amygdala neurons during associative learning
Visualizing stimulus convergence in amygdala neurons during associative learning
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DOI：
10.1073/pnas.0808996106
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发表时间：
2008-12-30
影响因子：
11.1
通讯作者：
Bernstein, Ilene L.
中科院分区：
文献类型：
--
作者：
Barot, Sabiha K.;Kyono, Yasuhiro;Bernstein, Ilene L.
联想记忆形成模型的一个核心特征是依赖于对条件刺激（CS）和非条件刺激（US）作出反应的通路的信息收敛。特别是，接收一致输入的细胞被认为对随后的可塑性至关重要。然而，在哺乳动物的大脑中，在学习事件中对这种一致输入作出反应的神经元的识别仍然是难以捉摸的。在这里，我们利用荧光原位杂交（catFISH）对时间基因转录进行Arc细胞区室分析，以定位哺乳动物大脑中在一次试验学习任务条件性味觉厌恶（CTA）训练中对CS和US都有反应的神经元群体。杏仁核基底外侧核（BLA）的单个神经元在条件反射过程中对CS味道和US药物都有反应。然而，当刺激物暴露被改变而对促进学习无效时（后向条件反射、潜伏抑制），同步激活并不明显。综上所述，这些数据提供了哺乳动物大脑中神经元在习得学习关联过程中接收CS和US的收敛信息的清晰可视化。
A central feature of models of associative memory formation is the reliance on information convergence from pathways responsive to the conditioned stimulus (CS) and unconditioned stimulus (US). In particular, cells receiving coincident input are held to be critical for subsequent plasticity. Yet identification of neurons in the mammalian brain that respond to such coincident inputs during a learning event remains elusive. Here we use Arc cellular compartmental analysis of temporal gene transcription by fluorescence in situ hybridization (catFISH) to locate populations of neurons in the mammalian brain that respond to both the CS and US during training in a one-trial learning task, conditioned taste aversion (CTA). Individual neurons in the basolateral nucleus of the amygdala (BLA) responded to both the CS taste and US drug during conditioning. Coincident activation was not evident, however, when stimulus exposure was altered so as to be ineffective in promoting learning (backward conditioning, latent inhibition). Together, these data provide clear visualization of neurons in the mammalian brain receiving convergent information about the CS and US during acquisition of a learned association.