The calcium sensor TaCBL4 and its interacting protein TaCIPK5 are required for wheat resistance to stripe rust fungus
The calcium sensor TaCBL4 and its interacting protein TaCIPK5 are required for wheat resistance to stripe rust fungus
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DOI：
10.1093/jxb/ery227
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发表时间：
2018-08-17
影响因子：
6.9
通讯作者：
Guo, Jun
中科院分区：
文献类型：
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作者：
Liu, Peng;Duan, Yinghui;Guo, Jun
钙调磷酸酶B样蛋白（CBL）作为钙离子传感器激活特异性蛋白激酶，即CBL相互作用蛋白激酶（CIPKs）。最近的研究表明，CBL-CIPK复合物不仅是非生物胁迫信号所必需的，而且还可能参与生物胁迫感知。然而，这种复合物在免疫信号传导中的作用，包括病原体感知，是未知的。在本研究中，我们分离了TaCBL-TaCIPK复合物的一个信号组分（TaCBL 4-TaCIPK 5），并表征了其在小麦（Triticum aestivum）和条锈菌（Pucciniastriiformis f.）条锈菌（Pst，stripe rust fungus）。在所有TaCBLs中，TaCBL 4 mRNA的积累在感染Pst后显著增加。TaCBL 4的沉默导致对无毒Pst感染的易感性增强。此外，筛选确定TaCIPK 5与TaCBL 4在植物中物理互作，并对小麦对Pst的抗性做出积极贡献。此外，TaCBL 4和TaCIPK 5共沉默的植物的抗病表型与单敲低植物的一致。在Pst感染过程中，所有沉默的植物中活性氧（ROS）的积累都发生了显着变化。总之，这些发现表明TaCBL 4-TaCIPK 5复合物以ROS依赖的方式正向调节小麦抗性，并为CBL-CIPK在小麦中的作用提供了新的见解。
Calcineurin B-like proteins (CBLs) act as Ca2+ sensors to activate specific protein kinases, namely CBL-interacting protein kinases (CIPKs). Recent research has demonstrated that the CBL-CIPK complex is not only required for abiotic stress signaling, but is also probably involved in biotic stress perception. However, the role of this complex in immune signaling, including pathogen perception, is unknown. In this study, we isolated one signaling component of the TaCBL-TaCIPK complex (TaCBL4-TaCIPK5) and characterized its role in the interaction between wheat (Triticum aestivum) and Puccinia striiformis f. sp. tritici (Pst, stripe rust fungus). Among all TaCBLs in wheat, TaCBL4 mRNA accumulation markedly increased after infection by Pst. Silencing of TaCBL4 resulted in enhanced susceptibility to avirulent Pst infection. In addition, screening determined that TaCIPK5 physically interacted with TaCBL4 in planta and positively contributed to wheat resistance to Pst. Moreover, the disease resistance phenotype of TaCBL4 and TaCIPK5 co-silenced plants was consistent with that of single-knockdown plants. The accumulation of reactive oxygen species (ROS) was significantly altered in all silenced plants during Pst infection. Together these findings demonstrate that the TaCBL4-TaCIPK5 complex positively modulates wheat resistance in a ROS-dependent manner, and provide new insights into the roles of CBL-CIPK in wheat.