Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains
Prevalence of Listeria monocytogenes in milk, meat and foodstuff of animal origin and the phenotype of antibiotic resistance of isolated strains
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DOI：
10.17221/5701-vetmed
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发表时间：
2004-07-01
影响因子：
0.7
通讯作者：
Klimova, E
中科院分区：
文献类型：
--
作者：
Navratilova, P;Schlegelova, J;Klimova, E
2000-2002年，对原料（牛奶和牛肉，518个样本）、即食食品和牛奶（30个和200个样本）和肉类（105个和110个样本）的最终产品以及两个肉类加工厂（41个样本）表面的拭子进行了检查，以确定是否存在单核细胞增生李斯特菌（李斯特菌）。单核细胞增多症）。采用标准微量稀释法测定70株分离菌对12种抗菌药物的敏感性，测定最低抑菌浓度（MIC）特征（MICm、MIC 50、MIC 90）。L.单核细胞增多症是从原料奶样品（15个样品）中分离的。除一份巴氏消毒奶样品外，未从任何半成品和最终乳制品中分离出该菌。此外，L。从生牛肉、肉糜、发酵干肉制品样品和生产设备的拭子中分离出单核细胞增多症。体外药敏试验表明，链霉素和诺氟沙星是最有效的（MICm = 4 μ g/ml），相当有效的检查抗菌物质。除了对克林霉素的中间抗性（MIC = 1-2 μ g/ml）和抗性（MIC = 4 μ g/ml）（分别为37和5株）外，所有菌株对测试物质均敏感。而L.特别是食品中的单核细胞增多症是严重的，目前对李斯特菌敏感性的测试。在捷克共和国，单核细胞增生分离株表明，由于来自食品的耐药菌株或耐药性转移到其他微生物而导致的不可治疗的感染的可能性很低。
In 2000-2002 samples of raw materials (milk and beef, 518 samples), ready-to-cook foods and final products from milk (30 and 200 samples) and from meat (105 and 110 samples) and swabs from surfaces in two meat-processing plants (41 samples) were examined for the presence of Listeria monocytogenes (L. monocytogenes). 70 isolates were tested using the standard microdilution method for the susceptibility to 12 antimicrobial drugs, minimum inhibitory concentration (MIC) characteristics (MICm, MIC50, MIC90) were determined. L. monocytogenes was isolated from raw milk samples (15 samples). It was not isolated from any of the semi-finished and final milk products except for one sample of pasteurized milk. Furthermore L. monocytogenes was isolated from samples of raw beef, meat emulsion, fermented dry meat products and from swabs from production equipment. In vitro testing of susceptibility showed the considerable effectiveness of examined antimicrobial substances with streptomycin and norfloxacin being least effective (MICm = 4 mug/ml). Apart from the intermediate resistance (MIC = 1-2 mug/ml) and resistance (MIC = 4 mug/ml) to clindamycin (37 and 5 strains, respectively), all strains were susceptible to the tested substances. While the presence of L. monocytogenes in foodstuffs, in particular, are serious, current tests of susceptibility of L. monocytogenes isolates indicate low probability untreatable infections as a result of resistant strains from foods or transfer of resistance to other microorganisms in the Czech Republic.