Purified Blueberry Anthocyanins and Blueberry Juice Alter Development of Obesity in Mice Fed an Obesogenic High-Fat Diet
Purified Blueberry Anthocyanins and Blueberry Juice Alter Development of Obesity in Mice Fed an Obesogenic High-Fat Diet
复制标题
DOI：
10.1021/jf902852d
复制
发表时间：
2010-04-14
影响因子：
6.1
通讯作者：
Howard, Luke R.
中科院分区：
文献类型：
--
作者：
Prior, Ronald L.;Wilkes, Samuel E.;Howard, Luke R.
雄性C57 BL/6 J小鼠（25日龄）喂食低脂饮食（10%千卡来自脂肪）（LF）或高脂饮食（45%千卡来自脂肪）（HF 45），持续72天。在LF或HF 45处理中包括饮用水中的蓝莓汁或纯化的蓝莓花青素（0.2或1.0mg/mL）。蔗糖被添加到一个治疗的饮用水中，以测试蓝莓汁中的糖是否会影响肥胖的发展。72天后，与LF喂养的小鼠相比，HF 45喂养的小鼠的总体重（g）和体脂（%）更高，而身体瘦肉组织（%）更低，但在喂食HF 45饮食加蓝莓汁或蓝莓花青素（0.2 mg/mL）的小鼠中，体脂（%）与喂食LF饮食的小鼠没有差异。花青素（ACN）降低腹膜后和附睾脂肪组织重量。在喂食HF 45饮食的小鼠中，空腹血清葡萄糖浓度较高。然而，在喂食HF 45饮食加上饮用水中0.2 mg ACN/mL的小鼠中，其降低至LF水平，但不含蓝莓汁。β细胞功能（HOMA-BCF）评分在喂食HF 45的情况下降低，但在喂食HF 45饮食加纯化的ACN（0.2 mg/mL）的小鼠中恢复到正常水平。喂食HF 45饮食的小鼠血清瘦素升高，并且喂食蓝莓汁或纯化的ACN（0.2mg/mL）相对于HF 45对照降低血清瘦素水平。饮用水中的蔗糖，当消费量被限制在所消耗的果汁的体积，产生较低的血清瘦素和胰岛素水平，瘦素/脂肪，腹膜后和总脂肪（%.BW）。蓝莓汁在预防肥胖方面不如饮用水中低剂量的花青素有效。需要进一步的研究来确定蓝莓汁和整个蓝莓在预防肥胖发展方面的不同反应的因素。
Male C57BL/6J mice (25 days of age) were fed either a low-fat diet (10% kcal from fat) (LF) or a high-fat diet (45% kcal from fat) (HF45) for a period of 72 days. Blueberry juice or purified blueberry anthocyanins (0.2 or 1.0 mg/mL) in the drinking water were included in LF or HF45 treatments. Sucrose was added to the drinking water of one treatment to test if the sugars in blueberry juice would affect development of obesity. Total body weights (g) and body fat (%) were higher and body lean tissue (%) was lower in the HF45 fed mice compared to the LF fed mice after 72 days, but in mice fed HF45 diet plus blueberry juice or blueberry anthocyanins (0.2 mg/mL), body fat (%) was not different from those mice fed the LF diet. Anthocyanins (ACNs) decreased retroperitoneal and epididymal adipose tissue weights. Fasting serum glucose concentrations were higher in mice fed the HF45 diet. However, it was reduced to LF levels in mice fed the HF45 diet plus 0.2 mg of ACNs/mL in the drinking water, but not with blueberry juice. beta cell function (HOMA-BCF) score was lowered with HF45 feeding but returned to normal levels in mice fed the HF45 diet plus purified ACNs (0.2 mg/mL). Serum leptin was elevated in mice fed HF45 diet, and feeding either blueberry juice or purified ACNs (0.2 mg/mL) decreased serum leptin levels relative to HF45 control. Sucrose in drinking water, when consumption was restricted to the volume of juice consumed, produced lower serum leptin and insulin levels, leptin/fat, and retroperitoneal and total fat (%.BW). Blueberry juice was not as effective as the low dose of anthocyanins in the drinking water in preventing obesity. Additional studies are needed to determine factors responsible for the differing responses of blueberry juice and whole blueberry in preventing the development of obesity.