Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype.
Dietary long-chain n-3 PUFAs increase LPL gene expression in adipose tissue of subjects with an atherogenic lipoprotein phenotype.
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发表时间：
2002-06
影响因子：
6.5
通讯作者：
Syrah H. Khan;A. Minihane;P. Talmud;John W. Wright;M. Murphy;Christine M. Williams;B. Griffin
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作者：
Syrah H. Khan;A. Minihane;P. Talmud;John W. Wright;M. Murphy;Christine M. Williams;B. Griffin
我们试图验证这一假设，即鱼油中的长链n-3 PUFA（LC n-3 PUFA）刺激人脂肪组织（AT）中脂蛋白脂酶（LPL）的基因表达。在一项随机、双盲、安慰剂对照、交叉研究中，51名表达致动脉粥样硬化脂蛋白表型（ALP）的男性受试者的饮食中补充鱼油6周。正如我们之前对该组的报道，补充LC n-3 PUFA可降低空腹血浆甘油三酯（TG）（-35%，P <0.05），减弱餐后TG反应（曲线下面积和增量面积; AUC和IAUC，P <0.05），并降低小而致密的LDL。本研究扩展了这些观察结果，显示这些变化伴随着脂肪组织中LPL mRNA浓度（AT-LPL mRNA，+55%，P = 0.003）和肝素后LPL活性（PH-LPL，+31%，P = 0.036）的显著增加。也有证据表明LPL基因表达和载脂蛋白E基因多态性之间存在关联。我们的结论是，饮食n-3 PUFA对ALP的有利影响可能是介导的，在一定程度上，通过增加血浆中的活性和人类脂肪组织中脂蛋白脂肪酶的基因表达。
We sought to test the hypothesis that dietary long-chain n-3 PUFA (LC n-3 PUFA) in fish oil stimulate the gene expression of lipoprotein lipase (LPL) in human adipose tissue (AT). In a randomized, double blind, placebo-controlled, cross-over study, 51 male subjects expressing an atherogenic lipoprotein phenotype (ALP) had their diets supplemented with fish oil for 6 weeks. As we previously reported for this group, supplementation with LC n-3 PUFA produced a decrease in fasting plasma triglyceride (TG) (-35%, P < 0.05), attenuation of the postprandial TG response (area and incremental area under the curve; AUC and IAUC, P < 0.05), and a decrease in small, dense LDL. The present study extended these observations by showing that these changes were accompanied by a marked increase in the concentration of LPL mRNA in adipose tissue (AT-LPL mRNA, +55%, P = 0.003) and post-heparin LPL activity (PH-LPL, +31%, P = 0.036). There was also evidence of an association between LPL gene expression and polymorphism in the apolipoprotein E gene. We conclude that the favorable influence of dietary n-3 PUFA on the ALP may be mediated, in part, through an increase in the plasma activity and gene expression of lipoprotein lipase in human adipose tissue.