Instability of v-src sequences in nonhuman primate tumors cultured in vitro.
Instability of v-src sequences in nonhuman primate tumors cultured in vitro.
复制标题
体外培养的非人灵长类肿瘤中 v-src 序列的不稳定性。
DOI：
10.1016/0042-6822(87)90358-8
复制
发表时间：
1987
期刊：
影响因子：
3.7
通讯作者：
Ogston,CW
中科院分区：
文献类型：
--
作者：
Marczynska,B;Gilles,PN;Ogston,CW
我们分析了劳斯肉瘤病毒（RSV）和各种衍生病毒诱导的狨猴肿瘤和转化的狨猴细胞的DNA。 Southern印迹杂交用于确定v-src基因序列的存在。我们未能在源自体内诱导的狨猴肿瘤或体外转化的狨猴细胞系的高传代细胞中检测到v-srcDNA。无法检测序列与培养物中回复回复体的选择无关，因为所有细胞系都保留了转化的形态，并且体外转化的细胞在移植到成年同种异体狨猴中后保留了诱导肉瘤的能力。相比之下，我们在体外转化后 32 至 60 天分析的细胞中检测到整合的原病毒。原病毒序列似乎与转化病毒相同，但显然不稳定并继续转座。
We have analyzed the DNA of marmoset tumors induced and marmoset cells transformed by Rous sarcoma virus (RSV) and derivative viruses of various types. Southern blot hybridization was used to determine the presence of v-srcgene sequences. We failed to detect v-srcDNA in high-passage cells derived from marmoset tumors inducedin vivoor from marmoset cell lines transformedin vitro. The inability to detectsrcsequences was not related to selection of revertants in culture, since all cell lines retained transformed morphology and cells transformedin vitroretained the ability to induce sarcomas after transplantation into adult allogeneic marmosets. By contrast, we detected integrated proviruses in cells analyzed 32 to 60 days afterin vitrotransformation. The proviral sequences appeared to be identical to the transforming virus but were apparently unstable and continued to transpose.