Screening for Differentially Expressed Proteins Relevant to the Differential Diagnosis of Sarcoidosis and Tuberculosis.
Screening for Differentially Expressed Proteins Relevant to the Differential Diagnosis of Sarcoidosis and Tuberculosis.
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筛选与结节病和结核病鉴别诊断相关的差异表达蛋白。
DOI：
10.1371/journal.pone.0132466
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li HP
中科院分区：
文献类型：
--
作者：
Du SS;Zhao MM;Zhang Y;Zhang P;Hu Y;Wang LS;Zhou Y;Li QH;Li Y;Du YK;He X;Li N;Yin ZF;Wei YR;Weng D;Li HP
在这项研究中，我们试图确定结节病或结核病患者血清中差异表达的蛋白质，并评估这些蛋白质作为结节病和结核阴性结核病鉴别诊断的标志物。利用蛋白质芯片，我们发现了3个蛋白质表现出结节病和结核病患者之间的差异表达。使用酶联免疫吸附试验（ELISA）验证这些蛋白质的表达升高，并通过免疫组织化学进一步证实。使用受试者工作特征（ROC）曲线、逻辑回归分析、平行和系列检验来评估蛋白质的诊断功效。筛选与结节病和结核病相关的细胞间粘附分子1（ICAM-1）和瘦素差异表达蛋白。使用ROC曲线，我们发现ICAM-1（临界值：57740 pg/mL）的曲线下面积（AUC）、敏感性和特异性分别为0.718、62.3%和79.5%，而瘦素（临界值：1193.186 pg/mL）的AUC、敏感性和特异性分别为0.763、88.3%和65.8%。Logistic回归分析显示，瘦素和ICAM-1联合诊断的AUC、敏感性和特异性分别为0.787、89.6%和65.8%，而瘦素、ICAM-1和体重指数（BMI）联合诊断的AUC、敏感性和特异性分别为0.837、90.9%和64.4%，具有最高的诊断价值。平行和系列试验表明，BMI-leptin与ICAM-1系列平行是最好的诊断方法，其敏感性和特异性分别为86.5%和73.1%。因此，我们的研究结果表明，ICAM-1和瘦素在结节病患者的血清和肉芽肿中的表达升高。ICAM-1和瘦素是诊断结节病和鉴别结节病与结核阴性肺结核的潜在指标。
In this study, we sought to identify differentially expressed proteins in the serum of patients with sarcoidosis or tuberculosis and to evaluate these proteins as markers for the differential diagnosis of sarcoidosis and sputum-negative tuberculosis. Using protein microarrays, we identified 3 proteins exhibiting differential expression between patients with sarcoidosis and tuberculosis. Elevated expression of these proteins was verified using the enzyme-linked immunosorbent assay (ELISA) and was further confirmed by immunohistochemistry. Receiver operating characteristic (ROC) curve, logistic regression analysis, parallel, and serial tests were used to evaluate the diagnostic efficacy of the proteins. Intercellular Adhesion Molecule 1(ICAM-1) and leptin were screened for differentially expressed proteins relevant to sarcoidosis and tuberculosis. Using ROC curves, we found that ICAM-1 (cutoff value: 57740 pg/mL) had an area under the curve (AUC), sensitivity, and specificity of 0.718, 62.3%, and 79.5% respectively, while leptin (cutoff value: 1193.186 pg/mL) had an AUC, sensitivity, and specificity of 0.763, 88.3%, and 65.8%, respectively. Logistic regression analysis revealed that the AUC, sensitivity, and specificity of combined leptin and ICAM-1 were 0.787, 89.6%, and 65.8%, respectively, while those of combined leptin, ICAM-1, and body mass index (BMI) were 0.837, 90.9%, and 64.4%, respectively, which had the greatest diagnostic value. Parallel and serial tests indicated that the BMI-leptin parallel with the ICAM-1 serial was the best diagnostic method, achieving a sensitivity and specificity of 86.5% and 73.1%, respectively. Thus, our results identified elevated expression of ICAM-1 and leptin in serum and granulomas of sarcoidosis patients. ICAM-1 and leptin were found to be potential markers for the diagnosis of sarcoidosis and differential diagnosis of sarcoidosis and sputum-negative tuberculosis.