N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection.
N-acetylcysteine induced quenching of red fluorescent oligonucleotide-stabilized silver nanoclusters and the application in pharmaceutical detection.
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DOI：
10.1016/j.aca.2013.07.037
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发表时间：
2013-09
影响因子：
6.2
通讯作者：
Xinyi Wang;Ruoyun Lin;Zhihan Xu;Hongduan Huang;Limei Li;Fen Liu;Na Li;Xiaoda Yang
中科院分区：
文献类型：
--
作者：
Xinyi Wang;Ruoyun Lin;Zhihan Xu;Hongduan Huang;Limei Li;Fen Liu;Na Li;Xiaoda Yang
本文报道了一种基于寡核苷酸保护银纳米晶体（Ag NCs）红色荧光猝灭法测定n -乙酰半胱氨酸（NAC）的新方法，量子产率为68.3±0.3%。该方法成功地用于NAC颗粒的测定，其线性范围为100 ~ 1200 nM (LOD为50 nM)，潜在共存物质的干扰最小。这是首次发现含硫醇化合物的猝灭性能遵循非线性Stern-Volmer曲线，表明其猝灭机制复杂，以静态猝灭为主，其中NAC部分取代了Ag NCs的dna模板。本研究拓展了银纳米团簇的分析应用，并对巯基化合物对Ag纳米碳荧光的猝灭机理有了更深入的了解。
In this work, we reported a new, simple and sensitive method for determination of N-acetylcysteine (NAC) based on quenching of the red fluorescence of oligonuleotide-protected silver nanoculsters (Ag NCs) with the quantum yield of 68.3 ± 0.3%. This method was successfully used for the assay of NAC granules presenting a linear range from 100 nM to 1200 nM (LOD of 50 nM) with minimal interferences from potential coexisting substances. It is for the first time that quenching performance of the thiol-containing compound was found to follow a non-linear Stern–Volmer profile, indicative of a complicated quenching mechanism with static quenching dominating, in which DNA-template of Ag NCs was partly replaced by NAC, as elucidated by spectral investigations. This study extended the analytical application of silver nanoclusters as well as provided a more insightful understanding of the quenching mechanism of thiol-compounds on the fluorescence of Ag NCs.