Salt Tolerance Improvement in Rice through Efficient SNP Marker-Assisted Selection Coupled with Speed-Breeding
Salt Tolerance Improvement in Rice through Efficient SNP Marker-Assisted Selection Coupled with Speed-Breeding
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DOI：
10.3390/ijms20102585
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发表时间：
2019-05-02
影响因子：
5.6
通讯作者：
Mitsui, Toshiaki
中科院分区：
文献类型：
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作者：
Rana, Md Masud;Takamatsu, Takeshi;Mitsui, Toshiaki
盐分严重限制了水稻的新陈代谢、生长和产量。提高当地种植的高产品种的耐盐性是一个缓慢的过程。本研究旨在通过快速育种培育耐盐水稻新种质。在这里，我们精确地渗入hst 1基因，通过单核苷酸多态性（SNP）标记辅助选择将耐盐性从凯金转移到高产的Yukinko-mai（WT）水稻。利用biotron快速育种技术，经过6代17个月的选育，获得了BC 3F 3群体YNU 31 -2-4。通过全基因组测序进行的高分辨率基因分型显示，BC 3F 2基因组与WT具有93.5%的相似性，并且仅固定了2.7%的供体亲本等位基因。对BC 3F 2变体的功能注释沿着田间评估数据表明，携带hst 1基因的YNU 31 -2-4植物在正常生长条件下具有与WT相似的农艺性状。YNU 31 -2-4幼苗经受盐胁迫（125 mM NaCl）有显着更高的存活率和增加的地上部和根系生物量比WT。在组织水平上，定量和电子探针分析表明，盐胁迫下YNU 31 -2-4幼苗避免了Na+在地上部的积累。盐胁迫下，YNU 31 -2-4植株表现出比WT显著更高的净CO2同化和更低的产量下降的改良表型。YNU 31 -2-4是一个潜在的候选新品种，在幼苗和生殖阶段高度耐盐胁迫，并可能在全球气候变化下保持产量。
Salinity critically limits rice metabolism, growth, and productivity worldwide. Improvement of the salt resistance of locally grown high-yielding cultivars is a slow process. The objective of this study was to develop a new salt-tolerant rice germplasm using speed-breeding. Here, we precisely introgressed the hst1 gene, transferring salinity tolerance from Kaijin into high-yielding Yukinko-mai (WT) rice through single nucleotide polymorphism (SNP) marker-assisted selection. Using a biotron speed-breeding technique, we developed a BC3F3 population, named YNU31-2-4, in six generations and 17 months. High-resolution genotyping by whole-genome sequencing revealed that the BC3F2 genome had 93.5% similarity to the WT and fixed only 2.7% of donor parent alleles. Functional annotation of BC3F2 variants along with field assessment data indicated that YNU31-2-4 plants carrying the hst1 gene had similar agronomic traits to the WT under normal growth condition. YNU31-2-4 seedlings subjected to salt stress (125 mM NaCl) had a significantly higher survival rate and increased shoot and root biomasses than the WT. At the tissue level, quantitative and electron probe microanalyzer studies indicated that YNU31-2-4 seedlings avoided Na+ accumulation in shoots under salt stress. The YNU31-2-4 plants showed an improved phenotype with significantly higher net CO2 assimilation and lower yield decline than WT under salt stress at the reproductive stage. YNU31-2-4 is a potential candidate for a new rice cultivar that is highly tolerant to salt stress at the seedling and reproductive stages, and which might maintain yields under a changing global climate.