Hepatitis B virus genotyping by enzyme-linked immunosorbent assay in Taiwan
Hepatitis B virus genotyping by enzyme-linked immunosorbent assay in Taiwan
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DOI：
10.1007/s12072-010-9198-y
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发表时间：
2010-09-01
影响因子：
6.6
通讯作者：
Chuang, Wan-Long
中科院分区：
文献类型：
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作者：
Liu, Shu-Fen;Hsieh, Meng-Hsuan;Chuang, Wan-Long
应用限制性片段长度多态(RFLP)和单抗酶联免疫吸附试验(ELISA法)对台湾地区人群进行HBV型别检测，并对其检测效率和精密度进行了评价。用酶联免疫吸附试验检测了100例慢性乙型病毒性肝炎患者的HBV型别，并与RFLP法检测的结果进行了比较。结果显示，本组病例中以B型为主(RFLP法为63%，ELISA法为62%)，其次为C型(RFLP法为31%，ELISA法为35%)。RFLP与ELISA法检测结果差异无统计学意义(P=0.75)。总的基因分型符合率、正确基因分型符合率和符合率分别为96.00%、94.79%和91.00%，B、C基因分型的符合率分别为96.77%、100.00%和96.77%。能同时进行基因分型的样本，平均HBVDNA水平较高(6.24+/-A1.77 vs.2.34+/-A0.90，logIU/ml)，且两种基因分型样本之间的HBVDNA水平有显著差异(P<；0.001)。在台湾地区，对于HBVDNA水平较低的患者，ELISA是一种实用且有用的基因分型方法。
Restriction fragment length polymorphism (RFLP) and enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies (mAbs) were used in this study to detect genotypes of HBV, and the efficiency and precision of ELISA using the mAbs for HBV genotype detection were also estimated.The ELISA with mAbs method was used for the detection of HBV genotype in a Taiwanese population. The HBV genotypes of 100 chronic hepatitis B patients were determined by ELISA and were then compared with those obtained using RFLP.Genotype B was found to be the most prevalent in this study (63% by RFLP; 62% by ELISA) followed by genotype C (31% by RFLP; 35% by ELISA). There was no significant difference between the results obtained by RFLP and ELISA (P = 0.75). The ELISA overall genotypeable rate, the correct genotyping rate from genotypeable specimens, and the concordance of the HBV genotyping assay was 96.00, 94.79, and 91.00%; for the ELISA HBV genotyping assay for genotype B specimens was 96.77, 100.00, and 96.77%; and for genotype C specimens was 97.14, 91.18, and 88.57%, respectively. The mean HBV DNA level was higher in the specimens that could be genotyped by both RFLP and ELISA samples (6.24 +/- A 1.77 vs. 2.34 +/- A 0.90, log IU/ml), and a significant difference in terms of HBV DNA level of more than 2 x 10(3) IU/ml was identified between the genotyped RFLP samples (P < 0.001).ELISA is a practical and a useful method for HBV genotyping in a clinical setting in Taiwan, in particular for patients with lower levels of HBV DNA.