Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes
Ubiquitin 7-amino-4-carbamoylmethylcoumarin as an improved fluorogenic substrate for deubiquitinating enzymes
复制标题
泛素 7-氨基-4-氨基甲酰甲基香豆素作为去泛素化酶的改良荧光底物
DOI：
10.1016/j.tet.2016.05.041
复制
发表时间：
2016-07-07
期刊：
影响因子：
2.1
通讯作者：
Li, Yi-Ming
中科院分区：
文献类型：
--
作者：
Li, Yi-Tong;Huang, Yi-Chao;Li, Yi-Ming
开发了一种新的荧光底物Ub-ACC(泛素C末端7-氨基-4-氨甲酰甲基香豆素)，用于DUB(脱泛素酶)活性测定。这种底物的合成效率高于经典的DUB底物Ub-AMC(泛素C端7-氨基-4-甲基香豆素)。UCH-L3、OTUD2和USP30的DUB检测表明，Ub-ACC的敏感性比Ub-AMC高近2倍。(C)2016爱思唯尔有限公司。保留所有权利。
A new fluorogenic substrate Ub-ACC (ubiquitin C-terminal 7-amino-4-carbamoylmethyl-coumarin) was developed for DUB (deubiquitinating enzyme) activity assays. This substrate can be synthesized with higher efficiency than the classical DUB substrate, Ub-AMC (ubiquitin C-terminal 7-amino-4-methylcoumarin). DUB assays using UCH-L3, OTUD2 and USP30 demonstrated that Ub-ACC shows nearly 2-fold higher sensitivity than Ub-AMC. (C) 2016 Elsevier Ltd. All rights reserved.