Effect of probiotic lozenges containing Lactobacillus reuteri on oral wound healing: a pilot study
Effect of probiotic lozenges containing Lactobacillus reuteri on oral wound healing: a pilot study
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DOI：
10.3920/bm2018.0003
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发表时间：
2018-01-01
影响因子：
5.4
通讯作者：
Pedersen, A. M. Lynge
中科院分区：
文献类型：
--
作者：
Twetman, S.;Keller, M. K.;Pedersen, A. M. Lynge
已经表明有益细菌可以刺激伤口愈合。目的是研究局部应用益生菌乳酸杆菌对标准化口腔伤口愈合的影响。本初步研究采用随机、安慰剂对照、双盲交叉设计。在10名健康志愿者的口腔粘膜中打孔标准化活检组织，暴露和不暴露于以锭剂和局部油施用的两种罗伊氏乳杆菌菌株。分别于术后2、5、8d进行临床评分。通过滤纸定量渗出物的量，并用多重免疫测定法测定所选细胞因子和趋化因子的水平。分别在活检前和愈合后采集唾液样本，用ELISA法测定催产素。通过每日日志报告主观感知的疼痛和不适。在第2天和第5天检查时，试验组有明显的愈合改善趋势，但与安慰剂干预相比差异无统计学意义（P=0.08）。与安慰剂组相比，益生菌组的伤口渗出液中肿瘤坏死因子（TNF）超家族配体成员13（APRIL）和13 B（BAFF）以及趋化因子白细胞介素8（IL-8）的表达更高，但不显著，特别是在5天和8天后。唾液中催产素水平显著低于对照组（P
It has been suggested that beneficial bacteria may stimulate wound healing. The aim was to investigate the effect of topical applications of probiotic lactobacilli on the healing of standardised oral wounds. This pilot study employed a randomised, placebo-controlled, double-blind cross-over design. Standardised biopsies were punched in the oral mucosa of 10 healthy volunteers, with and without exposure to two strains of Lactobacilli reuteri administrated as lozenges and topical oil. The healing was scored clinically after 2, 5 and 8 days. The amount of exudate was quantified through filter papers and the levels of selected cytokines and chemokines were determined with multiplex immunoassays. Saliva samples were collected before the biopsy and after healing for determination of oxytocin with ELISA. Subjectively perceived pain and discomfort was reported through a daily logbook. There was a clear tendency of improved healing in test group at the 2-and 5-day check-ups but the difference compared with the placebo intervention was not statistically significant (P=0.08). Higher but non-significant expressions of the tumour necrosis factor (TNF) superfamily ligand members 13 (APRIL) and 13B (BAFF), as well as the chemokine interleukin 8 (IL-8), were displayed in wound exudates from the probiotic group as compared with placebo, particularly after 5 and 8 days. The salivary levels of oxytocin were significantly lower (P