The duodenal microbiome is altered in small intestinal bacterial overgrowth
The duodenal microbiome is altered in small intestinal bacterial overgrowth
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DOI：
10.1371/journal.pone.0234906
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发表时间：
2020-07-09
期刊：
影响因子：
3.7
通讯作者：
Pimentel, Mark
中科院分区：
文献类型：
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作者：
Leite, Gabriela;Morales, Walter;Pimentel, Mark
小肠细菌过度生长(SIBO)非常普遍，并与许多胃肠道疾病有关，但涉及的微生物仍不清楚。此外，现有的关于SIBO微生物群变化的研究使用了粪便样本，这些样本不能代表整个胃肠道。因此，我们的目标是利用接受标准护理食道胃十二指肠镜检查而不做结肠准备的受试者的十二指肠抽吸物，确定并比较SIBO受试者和非SIBO受试者的十二指肠微生物组组成。使用最近重新定义的每毫升10(3)菌落形成单位(CFU/mL)的SIBO截止值，确定了42名SIBO和98名非SIBO受试者。SIBO患者的十二指肠标本在MacConkey琼脂上的计数是非SIBO患者的4x10(3)倍(P
Small intestinal bacterial overgrowth (SIBO) is highly prevalent and is associated with numerous gastrointestinal disorders, but the microbes involved remain poorly defined. Moreover, existing studies of microbiome alterations in SIBO have utilized stool samples, which are not representative of the entire gastrointestinal tract. Therefore, we aimed to determine and compare the duodenal microbiome composition in SIBO and non-SIBO subjects, using duodenal aspirates from subjects undergoing standard-of-care esophagogastroduodenoscopy without colon preparation. Using the recently-redefined cutoff for SIBO of >10(3)colony forming units per milliliter (CFU/mL), 42 SIBO and 98 non-SIBO subjects were identified. Duodenal samples from SIBO subjects had 4x10(3)-fold higher counts than non-SIBO subjects when plated on MacConkey agar (P