STIMULATION OF EMBRYONIC MOUSE LIMB BUD MESENCHYMAL CELL-GROWTH BY PEPTIDE GROWTH-FACTORS
STIMULATION OF EMBRYONIC MOUSE LIMB BUD MESENCHYMAL CELL-GROWTH BY PEPTIDE GROWTH-FACTORS
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DOI：
10.1002/jcp.1041120307
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发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
UNDERWOOD, LE
中科院分区：
文献类型：
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作者：
KAPLOWITZ, PB;DERCOLE, AJ;UNDERWOOD, LE
肽类生长因子在哺乳动物子宫内细胞生长中的可能作用是使用来自11天小鼠胚胎肢芽的未分化间充质细胞的原代培养物来研究的。当作为单层培养物生长时，高细胞密度非常有利于增殖。在含有0.2%血清的培养基中，纯化的表皮生长因子（EGF）、成纤维细胞生长因子（FGF）、增殖刺激活性（MSA）、胰岛素和生长调节素-C（Sm-C）不增加细胞生长，但小鼠胎肝条件培养基的30- 40，000 MW组分具有刺激性。当肢芽细胞作为高密度或微团培养物生长时，一种更好地接近体内生长条件的方法，所有测试的纯化生长因子都显著刺激细胞生长。当组合使用时，这些生长因子具有累加效应，用肝培养基（10%vol/vol）、EGF（10 ng/ml）、FGF（200 ng/ml）和胰岛素（1 μ g/ml）或Sm-C（20 ng/ml）观察到最佳刺激。肢芽细胞对生长刺激的反应受细胞培养方式的影响，并且需要至少4种不同的生长因子以实现最佳体外增殖。其中之一，肝介质的活性成分，似乎是一个以前未知的生长因子。
The possible role of peptide growth factors in mammalian intrauterine cell growth was investigated using primary cultures of undifferentiated mesenchymal cells from 11-day mouse embryo limb buds. When grown as monolayer cultures, proliferation is greatly favored by high cell densities. In medium containing 0.2% serum, purified epidermal growth factor (EGF), fibroblast growth factor (FGF), multiplication stimulating activity (MSA), insulin, and somatomedin-C (Sm-C) do not increase cell growth, but a 30-40,000 MW component of mouse fetal liver conditioned medium is stimulatory. When limb bud cells are grown as high density or micromass cultures, a method which better approximates in vivo growth conditions, all of the purified growth factors tested stimulate cell growth significantly. These growth factors have additive effects when used in combination, the best stimulation being observed with liver medium (10% vol/vol), EGF (10 ng/ml), FGF (200 ng/ml), and either insulin (1 .mu.g/ml) or Sm-C (20 ng/ml). The response of limb bud cells to growth stimulation is influenced by the manner in which the cells are cultured, and at least 4 different growth factors are required for optimal in vitro proliferation. One of these, the active component of liver medium, appears to be a previously uncharacterized growth factor.