REDUCED INHIBITORY ACTIVITY OF URONIC-ACID-RICH PROTEIN IN URINE OF STONE FORMERS
REDUCED INHIBITORY ACTIVITY OF URONIC-ACID-RICH PROTEIN IN URINE OF STONE FORMERS
复制标题
DOI：
10.1007/bf00541903
复制
发表时间：
1994-09-01
影响因子：
--
通讯作者：
DAUDON, M
中科院分区：
文献类型：
--
作者：
ATMANI, F;LACOUR, B;DAUDON, M
我们最近报道，人尿中含有一种新鉴定的尿糖蛋白，可有效抑制草酸钙结晶。这种抑制剂是一种富含糖醛酸的蛋白（UAP），分子量约为35 kDa。在本研究中，从结石形成者和无结石病史的受试者的尿液中分离出UAP，并在体外草酸钙结晶系统中测试其抑制活性。我们的研究结果表明，从结石形成者的尿液中提取的UAP比从健康受试者的尿液中提取的UAP的抑制活性弱。氨基酸和碳水化合物含量的初步分析表明，两组之间存在一些差异。主要区别是从结石形成者的尿液中分离的不稳定型心绞痛中唾液酸减少。我们认为，不稳定型心绞痛有助于显着的总尿草酸钙结晶的抑制活性，并在尿中的经常性结石形成者的这种活动的减少是由于，部分原因是由于不稳定型心绞痛的抑制活性弱。UAP的结构异常可以解释其在结石形成者尿液中抑制活性的减弱。
We recently reported that human urine contains a newly identified urinary glycoprotein acting as a potent inhibitor against calcium oxalate crystallization. This inhibitor is a uronic-acid-rich protein (UAP) with a molecular weight of approximately 35 kDa. In the present study, UAP was isolated from urine of stone formers and of subjects without a stone history, and its inhibitory activity was tested in a calcium oxalate crystallization system in vitro. Our results show a weaker inhibitory activity of UAP extracted from the urine of stone formers than that extracted from the urine of healthy subjects. Preliminary analyses of amino acid and carbohydrate content showed some differences between the two groups. The main difference was the reduction in sialic acid in UAP isolated from the urine of stone formers. We suggest that UAP contributes significantly to total urinary inhibitory activity of calcium oxalate crystallization and that the decrease in this activity in the urine of recurrent stone formers is due, in part, to the weak inhibitory activity of UAP. A structural abnormality of UAP could explain the diminution of its inhibitory activity in the urine of stone formers.