Impact of electronic cigarette heating coil resistance on the production of reactive carbonyls, reactive oxygen species and induction of cytotoxicity in human lung cancer cells in vitro
Impact of electronic cigarette heating coil resistance on the production of reactive carbonyls, reactive oxygen species and induction of cytotoxicity in human lung cancer cells in vitro
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DOI：
10.1016/j.yrtph.2019.104500
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发表时间：
2019-12-01
影响因子：
3.4
通讯作者：
Elias, Ryan J.
中科院分区：
文献类型：
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作者：
Cirillo, Silvia;Urena, Jose F.;Elias, Ryan J.
近年来，电子烟（e-cigarette; e-cig）的使用呈指数级增长，尽管它们对健康的影响尚不清楚。现在已知电子烟气溶胶含有有害的化学化合物，包括羰基化合物和活性氧物质（ROS），这些化合物在电子烟使用期间被消费者直接吸入。当液体与电子烟雾化器内的加热元件接触时，羰基化合物和ROS都会形成。在本研究中，线圈电阻（1.5 Ω和0.25 Ω线圈，分别获得8 +/-2 W和40 +/- 5 W的总瓦数）对羰基化合物（甲醛，乙醛，丙烯醛）和ROS的产生的影响进行了研究。还评价了由不同线圈产生的气溶胶对H1299人肺癌细胞活力的影响。我们的结果显示低电阻线圈与电子烟气溶胶中较高浓度的所选羰基化合物和ROS的产生之间存在显著（p < 0.05）相关性。此外，暴露于电子烟蒸汽使H1299细胞的活力降低高达45.8%，并且这种影响与线圈电阻呈负相关。尽管还需要进一步的研究来更好地阐明电子烟排放的潜在毒性，但我们的结果表明，这些设备可能会使用户接触有害化合物，从而可能会促进慢性呼吸道疾病。
Electronic cigarette (e-cigarette; e-cig) use has grown exponentially in recent years despite their unknown health effects. E-cig aerosols are now known to contain hazardous chemical compounds, including carbonyls and reactive oxygen species (ROS), and these compounds are directly inhaled by consumers during e-cig use. Both carbonyls and ROS are formed when the liquid comes into contact with a heating element that is housed within an e-cig's atomizer. In the present study, the effect of coil resistance (1.5 Omega and 0.25 Omega coils, to obtain a total wattage of 8 +/- 2W and 40 +/- 5 W, respectively) on the generation of carbonyls (formaldehyde, acetaldehyde, acrolein) and ROS was investigated. The effect of the aerosols generated by different coils on the viability of H1299 human lung carcinoma cells was also evaluated. Our results show a significant (p < 0.05) correlation between the low resistance coils and the generation of higher concentrations of the selected carbonyls and ROS in e-cig aerosols. Moreover, exposure to e-cig vapor reduced the viability of H1299 cells by up to 45.8%, and this effect was inversely related to coil resistance. Although further studies are needed to better elucidate the potential toxicity of e-cig emissions, our results suggest that these devices may expose users to hazardous compounds which, in turn, may promote chronic respiratory diseases.