Discrimination of leukemic Jurkat cells from normal lymphocytes via novo label-free cytometry based on fluctuation of image gray values
Discrimination of leukemic Jurkat cells from normal lymphocytes via novo label-free cytometry based on fluctuation of image gray values
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DOI：
10.1007/s00249-019-01351-w
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发表时间：
2019-04-01
影响因子：
2
通讯作者：
Deutsch, Mordechai
中科院分区：
文献类型：
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作者：
Wohl, Ishay;Zurgil, Naomi;Deutsch, Mordechai
我们介绍了一种简单的、无标记的细胞术技术，该技术基于灰度信息熵（GLIE）函数对细胞图像像素灰度的时空波动分析。本研究在白血病Jurkat细胞和人类健康供体淋巴细胞的比较细胞模型中，探讨GLIE随机波动及其生物物理病因的差异。使用了一种普通亮场显微镜和一种独特的成像盘的组合，其中细胞被单独地不受束缚地保存在一皮升体积矩阵的光学室中。恶性Jurkat细胞的随机GLIE波动大于良性淋巴细胞，而这些波动与细胞内囊泡均方位移（MSD）值相关，并被肌球蛋白-2和三磷酸腺苷（ATP）抑制剂抑制。这些结果表明，作用于细胞骨架上的非相干有源力引起细胞骨架和相关细胞内含量的机械耗散波动是GLIE随机波动结果背后的生物物理细胞机制。对Jurkat细胞和正常淋巴细胞结果的分析表明，这种简单且自动化的无标记细胞术可能具有识别恶性肿瘤的潜力，特别是在多细胞检查的诊断设置中。
We introduce a simple, label-free cytometry technique, based on the spatio-temporal fluctuation analysis of pixel gray levels of a cell image utilizing the Gray Level Information Entropy (GLIE) function. In this study, the difference in GLIE random fluctuations and its biophysical etiology in a comparison cell model of leukemic Jurkat cells and human healthy donor lymphocytes was explored. A combination of common bright field microscopy and a unique imaging dish wherein cells are individually held untethered in a picoliter volume matrix of optical chambers was used. Random GLIE fluctuations were found to be greater in malignant Jurkat cells than in benign lymphocytes, while these fluctuations correlate with intracellular vesicle Mean Square Displacement (MSD) values and are inhibited by myosin-2 and adenosine triphosphate (ATP) inhibitors. These results suggest that the incoherent active forces acting on the cytoskeleton which cause mechanical dissipative fluctuation of the cytoskeletal and related intracellular content are the biophysical cellular mechanism behind the GLIE random fluctuation results. Analysis of the results in Jurkat cells and normal lymphocytes suggests the possible potential of this simple and automated label-free cytometry to identify malignancy, particularly in a diagnostic setup of multiple cell examination.