Maternal Active Mastication during Prenatal Stress Ameliorates Prenatal Stress-Induced Lower Bone Mass in Adult Mouse Offspring.
Maternal Active Mastication during Prenatal Stress Ameliorates Prenatal Stress-Induced Lower Bone Mass in Adult Mouse Offspring.
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DOI：
10.7150/ijms.18298
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发表时间：
2017
影响因子：
3.6
通讯作者：
Kubo KY
中科院分区：
文献类型：
--
作者：
Azuma K;Ogura M;Kondo H;Suzuki A;Hayashi S;Iinuma M;Onozuka M;Kubo KY
慢性心理压力是骨质疏松症的危险因素。母体在产前压力下的主动咀嚼减弱了压力反应。本研究的目的是验证母体主动咀嚼影响产前应激对成年后代骨量和骨显微结构的影响这一假设。将妊娠ddY小鼠随机分为对照组、应激组和应激/咀嚼组。将应激和应激/咀嚼组小鼠置于通风约束管中45分钟，每天3次，并在妊娠第12天开始，持续至分娩。在束缚应激期间，允许应激/咀嚼组中的小鼠咀嚼木棍。使用定量显微CT、骨组织形态计量学和生化标志物评价5个月大雄性后代的骨反应。产前应激导致子代椎骨和股骨远端骨小梁量显著减少。母亲在产前应激期间的主动咀嚼减弱了骨形成减少和骨吸收增加，改善了由产前应激引起的后代骨小梁体积降低和骨显微结构恶化。这些发现表明，母亲在产前压力下的主动咀嚼可以改善产前压力引起的成年后代椎骨和股骨骨量降低。母鼠在产前应激期间的主动咀嚼可能是防止其后代骨量降低的有效应对策略。
Chronic psychological stress is a risk factor for osteoporosis. Maternal active mastication during prenatal stress attenuates stress response. The aim of this study is to test the hypothesis that maternal active mastication influences the effect of prenatal stress on bone mass and bone microstructure in adult offspring. Pregnant ddY mice were randomly divided into control, stress, and stress/chewing groups. Mice in the stress and stress/chewing groups were placed in a ventilated restraint tube for 45 minutes, 3 times a day, and was initiated on day 12 of gestation and continued until delivery. Mice in the stress/chewing group were allowed to chew a wooden stick during the restraint stress period. The bone response of 5-month-old male offspring was evaluated using quantitative micro-CT, bone histomorphometry, and biochemical markers. Prenatal stress resulted in significant decrease of trabecular bone mass in both vertebra and distal femur of the offspring. Maternal active mastication during prenatal stress attenuated the reduced bone formation and increased bone resorption, improved the lower trabecular bone volume and bone microstructural deterioration induced by prenatal stress in the offspring. These findings indicate that maternal active mastication during prenatal stress can ameliorate prenatal stress-induced lower bone mass of the vertebra and femur in adult offspring. Active mastication during prenatal stress in dams could be an effective coping strategy to prevent lower bone mass in their offspring.