Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans
Active defence responses associated with non-host resistance of Arabidopsis thaliana to the oomycete pathogen Phytophthora infestans
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DOI：
10.1046/j.1364-3703.2003.00195.x
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发表时间：
2003-11-01
影响因子：
4.9
通讯作者：
Kamoun, S
中科院分区：
文献类型：
--
作者：
Huitema, E;Vleeshouwers, VGAA;Kamoun, S
非寄主抗性或种特异性抗性的分子基础仍然是植物-微生物相互作用研究中的主要未知数之一。在本文中，我们描述了一个非主机的病理系统，涉及模式植物拟南芥和经济上重要的和破坏性的卵菌病原体致病疫霉的表征。这种相互作用的早期阶段的细胞学调查显示，萌发的A infestans孢囊拟南芥叶片，直接渗透的表皮细胞，形成感染囊泡，偶尔次生菌丝，其次是一个典型的过敏反应。使用动力学PCR监测侵染拟南芥期间的infestans生物量动态，揭示在接种后的第一个24小时期间生物量增加，随后在后期阶段减少。转基因报告细胞系和RNA印迹分析用于表征致病疫霉感染后诱导的防御反应。在接种后48小时观察到PDF1.2的显著诱导，而在接种后3天检测到PR基因表达水平升高。为了进一步表征这种防御反应，进行DNA微阵列分析以确定c. 11 000拟南芥cDNA感染后16小时。这些分析揭示了拟南芥非宿主反应和文献中描述的其他防御相关的治疗之间的显着重叠。特别是，非寄主对致病疫霉的反应显然与茉莉酸途径的激活有关。所描述的P. infestans致病系统为提高我们对非宿主抗性的理解提供了极好的前景。
The molecular basis of non-host resistance, or species-specific resistance, remains one of the major unknowns in the study of plant-microbe interactions. In this paper, we describe the characterization of a non-host pathosystem involving the model plant Arabidopsis thaliana and the economically important and destructive oomycete pathogen Phytophthora infestans. Cytological investigations into the early stages of this interaction revealed the germination of A infestans cysts on Arabidopsis leaves, direct penetration of epidermal cells, formation of infection vesicles and occasionally secondary hyphae, followed by a typical hypersensitive response. A infestans biomass dynamics during infection of Arabidopsis was monitored using kinetic PCR, revealing an increase in biomass during the first 24 h after inoculation, followed by a decrease in the later stages. Transgenic reporter lines and RNA blot analyses were used to characterize the defence responses induced following P. infestans infection. Significant induction of PDF1.2 was observed at 48 h after inoculation, whereas elevated levels of PR gene expression were detected three days after inoculation. To further characterize this defence response, DNA microarray analyses were carried out to determine the expression profiles for c. 11 000 Arabidopsis cDNAs 16 h after infection. These analyses revealed a significant overlap between Arabidopsis non-host response and other defence-related treatments described in the literature. In particular, non-host response to P. infestans was clearly associated with activation of the jasmonate pathway. The described Arabidopsis-P. infestans pathosystem offers excellent prospects for improving our understanding of non-host resistance.