Effect of chronic methanol administration on amino acids and monoamines in retina, optic nerve, and brain of the rat
Effect of chronic methanol administration on amino acids and monoamines in retina, optic nerve, and brain of the rat
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DOI：
10.1006/taap.2002.9477
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发表时间：
2002-12-01
影响因子：
3.8
通讯作者：
Lima, L
中科院分区：
文献类型：
--
作者：
González-Quevedo, A;Obregón, F;Lima, L
慢性甲醇中毒的临床和视网膜电图特征是稀缺的，神经递质的研究尚未进行。此外，该领域的大多数研究包括急性给药后的结果。在目前的工作中，慢性甲醇中毒计划（2 g/kg/天ip 2周）进行了Sprague-Dawley大鼠先前耗尽叶酸与甲氨蝶呤。还在两组动物中给予含牛磺酸（2%）的饮用水。与对照组相比，甲氨蝶呤-甲醇处理动物的血甲酸盐水平升高（分别为0.98+/-0.09和0.30+/-0.03 mM）。氨基酸和单胺测定血浆和视网膜，视神经，海马，和后皮质HPLC荧光或电化学检测。主要发现是甲氨蝶呤甲醇处理动物视神经中天冬氨酸含量增加。单独使用甲醇可增加海马中谷氨酸、天冬氨酸、谷氨酰胺、牛磺酸、5-羟色胺和5-羟吲哚乙酸的水平，并增加视网膜中5-羟色胺的水平。牛磺酸给药对甲醇处理诱导的变化无显著影响。我们的结论是，慢性甲醇管理产生积累的天冬氨酸，兴奋性毒性氨基酸，在视神经。这些发现有助于理解甲醇的神经毒性，并可能表明慢性甲醇消费和视神经病变的发展之间的关系。(C)2002 Elsevier Science（美国）。
The clinical and electroretinographic features of chronic methanol intoxication are scarce, and neurotransmitter studies have not been conducted. In addition, most of the studies in the field include results after acute administration. In the present work, a chronic methanol intoxication scheme (2 g/kg/day ip for 2 weeks) was carried out in Sprague-Dawley rats previously depleted of folates with methotrexate. Taurine (2%) in drinking water was also administered in two groups of animals. Blood formate levels were increased in methotrexate-methanol-treated animals with respect to controls (0.98+/-0.09 and 0.30+/-0.03 mM, respectively). Amino acids and monoamines were determined in plasma and in retina, optic nerve, hippocampus, and posterior cortex by HPLC with fluorescence or electrochemical detection. The main finding was an increased aspartate content in the optic nerve in methotrexate methanol-treated animals. Methanol alone increased glutamate, aspartate, glutamine, taurine, 5-hydroxytryptamine, and 5-hydroxyindoleacetic acid levels in the hippocampus and 5-hydroxytryptamine in the retina. Taurine administration had no significant effect on changes induced by methanol treatment. We concluded that chronic methanol administration produced accumulation of aspartate, an excitotoxic amino acid, in the optic nerve. These findings contribute to the understanding of methanol neurotoxicity and might indicate a relationship between chronic methanol consumption and the development of optic neuropathies. (C) 2002 Elsevier Science (USA).