Magnetic Actuator Device Assisted Modulation of Cellular Behavior and Tuning of Drug Release on Silk Platform
Magnetic Actuator Device Assisted Modulation of Cellular Behavior and Tuning of Drug Release on Silk Platform
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DOI：
10.1021/acsbiomaterials.8b00240
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Mandal, Biman B.
中科院分区：
文献类型：
--
作者：
Chouhan, Dimple;Mehrotra, Shreya;Mandal, Biman B.
已经发现外部施加的物理力和机械刺激对细胞是有益的，导致它们的信号传导或分化。此外，生物反应器和功能性生物材料已经基于这一原理设计，以在体外条件下调节细胞行为。在此，我们设计了一个磁致动器设备（MAD），以了解两种不同现象的基本响应：驱动对心肌细胞的影响和脉冲磁场的影响下的药物输送。丝素蛋白（SF）为基础的磁响应矩阵，通过将磁性氧化铁纳米粒子（IONP）的丝纳米纤维内的开发与MAD驱动。将细胞和药物分别接种到丝素基质中，研究MAD物理驱动对细胞行为和药物释放特性的影响。以新生大鼠心肌细胞和H9c2细胞为研究对象，以模型药物为观察对象。脉冲磁刺激促进细胞增殖，与静态条件下的细胞相比，其增殖率显著升高，p
Externally applied physical forces and mechanical stimulations have been found to be instructive to cells which lead to their signaling or differentiation. Further, bioreactors and functional biomaterials have been designed based on this principle to modulate cellular behavior under in vitro conditions. Herein, we have designed a magnetic actuator device (MAD) to understand the fundamental responses of two different phenomena: the effect of actuation on cardiac muscle cells and drug delivery under the influence of pulsed magnetic field. Silk fibroin (SF)-based magnetically responsive matrix, developed by incorporating magnetic iron oxide nanoparticles (IONP) within silk nanofibers was actuated with MAD. The silk matrix was seeded with cells and drugs independently to study effect of physical actuation by MAD on cellular behavior and drug release properties. Neonatal rat cardiomyocytes and H9c2 cells were used for studying the former while model drug was used to observe the latter. Pulsed magnetic stimulation promoted proliferation of cells, at a significantly higher rate in comparison to those under static conditions, p